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LINC00312 inhibits the migration and invasion of bladder cancer cells by targeting miR-197-3p

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Tumor Biology

Abstract

To investigate the influence of the long non-coding RNA LINC00312 on bladder cancer (BC) cell invasion and metastasis by targeting miR-197-3p. BC and corresponding adjacent tissues were collected. LINC00312 and miR-197-3p were measured, and their correlation was detected through quantitative real-time PCR (qRT-PCR). BC cell line T24 was transfected and grouped (five groups) according to different transfection conditions. A scratch test was applied to analyze cell migration, and a Transwell assay was used to test cell invasion ability. Western blotting was to measure matrix metalloproteinase (MMP)-2, MMP-9, and the tissue inhibitor of metalloproteinase 2 (TIMP2) protein levels. qRT-PCR indicated that LINC00312 expression was lower but miR-197-3p expression was higher in BC tissues compared with adjacent tissues; LINC00312 was negatively correlated with miR-197-3p. The migration test revealed that the downregulation of miR-197-3p and overexpression of LINC00312 inhibited cell migration and invasion abilities, while the overexpression of miR-197-3p and the upregulation of LINC00312 promoted cell migration and invasion. BC cells with downregulated miR-197-3p or upregulated LINC00312 had low MMP-2 and MMP-9 levels but high TIMP2. LINC00312 inhibited BC cell invasion and metastasis through mediating miR-197-3p.

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Acknowledgments

This study was supported by the Jiangsu Planned Projects for Postdoctoral Research Fund (1501142B). We would like to give our sincere appreciation to the reviewers for their helpful comments on this article.

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Authors and Affiliations

  1. Department of Urology, Huai’an First People’s Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai’an, Jiangsu Province, People’s Republic of China

    Yun-Yan Wang, Gong-Cheng Wang, Kun Liu, Xiao-Bing Niu, Shuo Gu & Jun-Song Meng

  2. Department of Urology, Huai’an Hospital affiliated of Xuzhou Medical College, Huai’an, 223002, People’s Republic of China

    Zi-Yu Wu

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  1. Yun-Yan Wang
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Correspondence to Jun-Song Meng.

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Yun-Yan Wang, Zi-Yu Wu, and Gong-Cheng Wang are regarded as co-first authors.

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Wang, YY., Wu, ZY., Wang, GC. et al. LINC00312 inhibits the migration and invasion of bladder cancer cells by targeting miR-197-3p. Tumor Biol. 37, 14553–14563 (2016). https://doi.org/10.1007/s13277-016-5303-8

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  • DOI: https://doi.org/10.1007/s13277-016-5303-8

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