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Association of a p73 exon 2 G4C14-to-A4T14 polymorphism with risk of hepatocellular carcinoma in a Chinese population

  • Research Article
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Tumor Biology

Abstract

P73, a p53 homolog, has some p53-like activities and plays an important role in modulating cell cycle, apoptosis, and DNA repair. A potentially functional dinucleotide polymorphism, G4C14-to-A4T14, has been identified in the 5′ untranslated region of exon 2 of the p73 gene, which may theoretically form a stem-loop structure and affect gene expression. We hypothesized that genetic variants in p73 may modify individual susceptibility to hepatocellular carcinoma (HCC). To test this hypothesis that these two common variants play a role in HCC susceptibility, we conducted a hospital-based case–control study of 476 HCC patients and 526 cancer-free controls in a Chinese population. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry method was performed to detect these polymorphisms. The results showed that the genotype and allele frequencies of the p73 G4C14-A4T14 did not differ significantly between the HCC patients and the control group (all P values are above 0.05). However, with stratification analysis by age, sex, smoking status, drinking status, HBV carrier state, and family history of cancer, we found that the variant genotypes (GC/AT + AT/AT) of the p73 G4C14-A4T14 was associated with a significant increased risk of HCC among HbsAg-positive individuals (adjusted OR = 2.19, 95 % CI = 1.25–3.83) and among women (adjusted OR = 2.62, 95 % CI = 1.47, 4.66). These results suggest that the p73 G4C14-to-A4T14 dinucleotide polymorphism may play a role in the development of chronic HBV-infected HCC in the Chinese population, especially among women.

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Abbreviations

HCC:

Hepatocellular carcinoma

HBV:

Hepatitis B virus

OR:

Odds ratio

CI:

Confidence interval

SNP:

Single nucleotide polymorphisms

HWE:

Hardy–Weinberg equilibrium

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (no. 81172372 and no.30901720) and Ph.D. Programs Foundation of the Ministry of Education of China (no. 20090181120111).

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Authors and Affiliations

  1. Division of Liver Transplantation, Department of Liver and Vascular Surgery, West China Hospital, Sichuan University, 37 Guo Xue Road, Chengdu, 610041, Sichuan Province, China

    Benyuan Deng, Fei Liu, Yonggang Wei, Xi Chen, Lvnan Yan & Bo Li

  2. Department of Clinical Immunological Laboratory, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China

    Limei Luo

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  1. Benyuan Deng
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  2. Fei Liu
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  7. Bo Li
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Correspondence to Bo Li.

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Deng, B., Liu, F., Wei, Y. et al. Association of a p73 exon 2 G4C14-to-A4T14 polymorphism with risk of hepatocellular carcinoma in a Chinese population. Tumor Biol. 34, 293–299 (2013). https://doi.org/10.1007/s13277-012-0550-9

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  • DOI: https://doi.org/10.1007/s13277-012-0550-9

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