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Neuroprotective effect of green tea extractives against oxidative stress by enhancing the survival and proliferation of PC12 cells

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Abstract

Backgrounds

Previous studies were mainly focused on the effect of catechins and caffeine, the main pharmacological ingredients of green tea. However, the ordinary way of taking green tea is by drinking the tea water. Hence the benefits of water extractives of green tea were investigated in the current study.

Methods

H2O2 was applied to induce the oxidative stress on PC12 cells. The neuroprotective effects of green tea against oxidative stress were determined by observing the biological behaviors of PC12 cells.

Results

With the pre-treatment of light green tea, the survival and proliferation of oxidative stressed PC12 cells were significantly enhanced. H2O2 induced PC12 cell apoptosis was completely reversed. Consistently, the expression of JNK was up-regulated while the caspase-3 was down-regulated.

Conclusion

These results suggested that drinking green tea, particularly the light green tea, performed neuroprotective effects against oxidative stress.

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (81571205 and 81870981).

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Authors and Affiliations

  1. Department of Neurobiology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an, Shannxi, P.R. China, 710061

    Zhenlu Cai, Xiaoxuan Hu, Ruolan Tan, Meiqi Sun, Ning Ma, Xingxing Li, Li Huang, Jing An, Qian Ge & Haixia Lu

  2. Department of Human Anatomy and Histo-embryology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an, Shannxi, P.R. China, 710061

    Xiaoxuan Hu, Ruolan Tan & Ning Ma

  3. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P.R. China, 300072

    Yunran Feng

Authors
  1. Zhenlu Cai
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  2. Xiaoxuan Hu
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  3. Ruolan Tan
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  4. Yunran Feng
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  5. Meiqi Sun
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  8. Li Huang
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  9. Jing An
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  10. Qian Ge
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  11. Haixia Lu
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Corresponding authors

Correspondence to Qian Ge or Haixia Lu.

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Conflict of Interest

Zhenlu Cai, Xiaoxuan Hu, Ruolan Tan, Yunran Feng, Meiqi Sun, Ning Ma, Xingxing Li, Li Huang, Jing An, Qian Ge and Haixia Lu declare that they have no conflict of interest.

Human and animal rights

The article does not contain any studies with human and animal and this study was performed following institutional and national guidelines.

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Cai, Z., Hu, X., Tan, R. et al. Neuroprotective effect of green tea extractives against oxidative stress by enhancing the survival and proliferation of PC12 cells. Mol. Cell. Toxicol. 15, 391–397 (2019). https://doi.org/10.1007/s13273-019-0042-8

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  • DOI: https://doi.org/10.1007/s13273-019-0042-8

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