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Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro

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Abstract

Backgrounds

While a few virions of hepatitis B virus (HBV) are sufficient to liver infection in vivo, even a large quantity of virion is unable to enter into hepatoma cells in vitro.

Methods

To solve this paradox, we explored if humoral milieu such as serum or culture media, and its constituents, and pH would regulate the viral DNA and surface antigen expression of HBV in vitro.

Results

The viral DNA and surface antigen expression of HBV are significantly more stable in human serum than in mouse serum, and Dulbecco’s Modified eagle Medium (DMEM). In line, the increase of anti-HBcAg antibody in mouse serum, and DMEM implies that the virion was cleared in non-host humoral milieu. Further, lipid analysis revealed higher levels of lipids in human serum than those in mouse serum, and DMEM. Lipid removal analysis showed the decreased level of HBV DNA and surface antigen expression in human and mouse serum. In electrolytes analysis, human serum contained lower level of potassium than mouse serum. Last, on the viral DNA and surface antigen expression of HBV, the alkalinity of DMEM was more effective than neutral pH.

Conclusion

Collectively, this study indicates that humoral milieu would regulate the viral DNA and surface antigen expression of HBV in vitro. This is the first report to regulate HBV growth/viability via simple manipulation of host humoral milieu.

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Authors and Affiliations

  1. Department of Biotechnology, Yonsei University College of Life Science and Biotechnology, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea

    Hong-Jai Lee, Bo-Young Shin, Jae-Seung Moon, Chun-Chang Ho, Jin-Su Shin, Jee-Sang Yoon, Jee-Eun Yang & Sang-Kyou Lee

  2. Department of Microbiology, Wonju College of Medicine, Yonsei University, 20 Ilsan-ro, Wonju, 26426, Republic of Korea

    Soo-Ki Kim

  3. Good T cells, Inc, Yonsei-ro 50 Seodaemun-gu, Seoul, Republic of Korea

    Sang-Kyou Lee

Authors
  1. Hong-Jai Lee
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  2. Bo-Young Shin
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  3. Jae-Seung Moon
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  4. Chun-Chang Ho
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  5. Jin-Su Shin
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  6. Jee-Sang Yoon
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  7. Jee-Eun Yang
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  8. Soo-Ki Kim
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  9. Sang-Kyou Lee
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Corresponding authors

Correspondence to Soo-Ki Kim or Sang-Kyou Lee.

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Lee, HJ., Shin, BY., Moon, JS. et al. Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro. Mol. Cell. Toxicol. 15, 123–128 (2019). https://doi.org/10.1007/s13273-019-0015-y

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  • DOI: https://doi.org/10.1007/s13273-019-0015-y

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