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Identification of differentially expressed miRNAs after Lactobacillus reuteri treatment in the ileum mucosa of piglets

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Abstract

Background

Lactobacillus reuteri I5007 possesses many excellent probiotic characteristics in piglets. miRNA plays important role in host-microbiota interactions, but the mechanism by which L. reuteri I5007 regulates intestinal function through its influence on miRNA expression is unknown.

Objective

This study analyzed the miRNA expression patterns in the ileum mucosa tissue of piglets by L. reuteri I5007 treatment, aim to clarify its molecular mechanism for regulating intestinal function through miRNA.

Methods

Neonatal piglets were orally administered L. reuteri I5007 or a placebo daily starting on day 1, and differential expression of ileal miRNAs was analyzed at 10 and 20 days of age by small RNA sequencing.

Results

361 known porcine miRNAs were identified, and ten miRNAs were highly expressed in the ileum mucosa in both treatments. Nineteen differentially expressed (DE) miRNAs were identified in response to L. reuteri treatment, and four DE miRNAs (ssc-miR-196a, -196b-5p, -1285 and -10386) were differentially expressed at both time points. The KEGG pathway analyses showed the targets of 19 DE miRNAs were involved in 63 significantly enriched pathways, including the PI3K-Akt and MAPK pathways, which were confirmed to play important roles in probiotic-host communication. L. reuteri I5007 exerted anti-inflammatory effects by influencing the levels of inflammatory cytokines. Suppressor of cytokine signalling 4 gene was the target gene of ssc-miR-196a/-196b-5p, overexpression of ssc-miR-196a/-196b-5p downregulated the mRNA expression of IL-1β and TNFα in IPEC-J2 cells.

Conclusion

Our study provides new insight into the role of miRNAs in the intestinal function of piglets after L. reuteri I5007 treatment.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant number: 31702151) and the National Key Research and Development Program of China (Grant number: 2017YFD0500506).

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Authors and Affiliations

  1. Chongqing Academy of Animal Sciences, Rongchang, Chongqing, 402460, China

    Qi Wang, Qian Sun, Jing Wang, Xiaoyu Qiu, Renli Qi & Jinxiu Huang

  2. Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing, 402460, China

    Qi Wang, Jing Wang, Renli Qi & Jinxiu Huang

  3. Chongqing Key Laboratory of Pig Industry Sciences, Rongchang, Chongqing, 402460, China

    Qi Wang, Jing Wang, Renli Qi & Jinxiu Huang

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  1. Qi Wang
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  2. Qian Sun
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  3. Jing Wang
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  4. Xiaoyu Qiu
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Correspondence to Jinxiu Huang.

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All animal protocols used in this study were in accordance with the Guidelines for the Administration of Affairs Concerning Experimental Animals, and the Institute Ethics Committee of the Chongqing Academy of Animal Science approved the relevant ethical issues (approval number: xky-20190218).

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13258_2020_998_MOESM1_ESM.rar

Supplementary file1 Table S1. Distribution of small RNAs in each libraries. Table S2. The read counts of known porcine miRNAs. Table S3. Significantly enriched KEGG pathways for target genes of 19 DE miRNAs. Fig. S1. The length distribution of small RNAs. Fig. S2. The top 10 highly expressed miRNAs in the ileum mucosa of piglets. Fig. S3. The PI3K-Akt signalling pathway was enriched for target genes of 19 DE miRNAs. Fig. S4. The MAPK signalling pathway was enriched for target genes of 19 DE miRNAs. (RAR 557 kb)

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Wang, Q., Sun, Q., Wang, J. et al. Identification of differentially expressed miRNAs after Lactobacillus reuteri treatment in the ileum mucosa of piglets. Genes Genom 42, 1327–1338 (2020). https://doi.org/10.1007/s13258-020-00998-6

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