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Identification and characterization of BGL11(t), a novel gene regulating leaf-color mutation in rice (Oryza sativa L.)

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Abstract

A novel bright-green leaf mutant, bgl11, derived from Nipponbare (Oryza sativa L. ssp. japonica) treated by ethyl methanesulfonate (EMS), exhibited a distinct bright-green leaf phenotype throughout development. Chlorophyll contents of bgl11 decreased significantly than that of its wild-type parent. Genetic analysis suggested that the bright-green leaf trait was controlled by a single recessive nuclear gene, which was tentatively designed as BGL11(t). To isolate the BGL11(t) gene, a map-based cloning strategy was employed, and the gene was finally mapped in a 94.7 kb region between marker InDel11-5 and InDel11-9 on the long arm of chromosome 11, in which no gene leaded to leaf-color mutation had been mapped or cloned. Cloning and sequencing analysis revealed that, LOC_Os11g38040, which was predicted to encode an expressed protein, had a 9 bp segment deletion in the coding region of bgl11. Furthermore, the transgenic plants with wild-type gene LOC_Os11g38040 were restored to normal phenotype. Accordingly, the gene (LOC_Os11g38040) was identified as the BGL11(t) gene. These results are very valuable for further study on BGL11(t) gene and illuminating the mechanism of chloroplast development in rice.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31070440), the Zhejiang Provincial Science and Technology of major Science and Technology Project (2008C12055) and the Innovation Research Team of Young Teachers of Zhejiang A & F University (No.2010RC02).

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Authors and Affiliations

  1. Institute of Applied Chemistry, Zhejiang Agriculture and Forestry University, Lin’an, 311300, Zhejiang, China

    Zhi-kun Wang, Zheng-diao Miao, Zhi-yan Hu & Li Liu

  2. College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Yun-xiang Huang

  3. School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin’an, 311300, Zhejiang, China

    Xin-zhang Song

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  1. Zhi-kun Wang
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  2. Yun-xiang Huang
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  3. Zheng-diao Miao
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  4. Zhi-yan Hu
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  5. Xin-zhang Song
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  6. Li Liu
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Correspondence to Zhi-kun Wang.

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Wang, Zk., Huang, Yx., Miao, Zd. et al. Identification and characterization of BGL11(t), a novel gene regulating leaf-color mutation in rice (Oryza sativa L.). Genes Genom 35, 491–499 (2013). https://doi.org/10.1007/s13258-013-0094-4

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