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An effective micropropagation protocol and determination of the clonal fidelity of in vitro developed microshoots of carnation (Dianthus caryophyllus L.) using SSR markers

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Abstract

Carnation is an important cut flower in the floriculture industry. A micropropagation method for carnation cv. ‘Irene’ has been standardized. Nodal plant explants were grown in Murashige and Skoog (MS) medium containing plant growth regulators (PGRs). Determination of clonal fidelity of regenerated plantlets was performed with the help of SSR markers. Early formation of micro-shoots with maximum regeneration was noted in the half MS medium in combination of benzyl amino purine (BAP) thidiazuron (TDZ) and silver nitrate (AgNO3) in the concentrations 1.5, 0.25 and 0.5 mg L−1, respectively; while BAP (2.5 mgL−1), TDZ (1.0 mg L−1) and AgNO3 (1.5 mg L−1) produced maximum length of the micro-shoots. Quick induction of roots and maximum number of roots per culture were achieved in half MS basal rooting medium supplemented with 1.5 mg L−1 of indole-3-butyric acid (IBA) and 0.5 mg L−1 of naphthalene acetic acid (NAA). Simple sequence repeat (SSR) markers for clonal fidelity analysis showed uniform genetic fidelity among the proliferated plantlets and the mother plant lines. The protocol can be suitable for commercial cultivation of carnation plants.

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Abbreviations

BAP:

Benzyl Amino Purine

TDZ:

Thidiazuron

AgNO3 :

Silver Nitrate

RAPD:

Randomly Amplified Polymorphic DNA

SSR:

Simple Sequence Repeats

ISSR:

Inter Simple Sequence Repeats

CTAB:

Cetyl Triethyl Ammonium Bromide

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Acknowledgements

Authors are highly thankful to Director Research, SVP University of Agriculture and Technology Meerut for providing all the necessary help towards conducting the experiments. We are also thankful to Director Research, Dr. YS Parmar University, Solan, Himachal Pradesh for giving us carnation germplasm used in this study.

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Author notes

  1. Priya

    Present address: Institute of Chemical Technology (ICT), Mumbai, India

Authors and Affiliations

  1. Department of Horticulture, College of Agriculture, SVP University of Agriculture & Technology, Meerut, 250 110, Uttar Pradesh, India

    Ram Lakhan Maurya & Mukesh Kumar

  2. Department of Biotechnology, SVP University of Agriculture & Technology, Meerut, 250 110, Uttar Pradesh, India

    Ujjwal Sirohi & Manoj Kumar Yadav

  3. College of Biotechnology, SVP University of Agriculture & Technology, Meerut, 250 110, Uttar Pradesh, India

    Priya

  4. Department of Chemistry, Meerut College Meerut, Uttar Pradesh, Meerut, India, 250003

    Veena Chaudhary

  5. Plant Genetic Resources and Improvement, CSIR-National Botanical Research Institute, Lucknow, 226 001, India

    Vinukonda Rakesh Sharma

  6. CSIR-National Botanical Research Institute, Lucknow, India

    Subodh Kumar Datta

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  1. Ram Lakhan Maurya
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All the authors contributed the research equally.

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Correspondence to Manoj Kumar Yadav.

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Maurya, R.L., Kumar, M., Sirohi, U. et al. An effective micropropagation protocol and determination of the clonal fidelity of in vitro developed microshoots of carnation (Dianthus caryophyllus L.) using SSR markers. Nucleus 65, 49–55 (2022). https://doi.org/10.1007/s13237-021-00362-3

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