Abstract
Two asynaptic plants were identified during meiosis from 0.2 % colchicine treated inflorescences for 12 h in Allium cepa. The asynaptic behavior could be noticed in early as well as at late diakinesis / metaphase-I. The chiasma frequency was very low as compared to control. At telophase-I/II, univalents created high abnormality. Unequal distributions, laggards, tripolar and micronuclei were found in asynaptic plants. Pollen fertility was found to be very low (55.1 and 52.6 %). Observations suggest that colchicine treatment impacts the meiosis of Allium cepa, suggesting that chromosomal associations in asynaptic plants are independent of meiotic behavior in the control.
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Darlington CD, Hague A. The timing of mitosis and meiosis in Allium ascalonicum. A problem of differentiation. Heredity. 1955;9:117–27.
El-Morsy SI, Dorra MDM, Elham AA, El-Hady A, Atef AAH, Ahmed YM. Comparative studies on diploid and tetraploid levels of Nicotiana alata. Acad J Pl Sci. 2009;2:182–8.
Fedotova YS, Bogdanov YF, Gadzhiyeva SA, Sosnikhina SA, Smirnov VG, Mikhailova EI. Meiotic mutants of rye Secale cereale II. The nonhomologous synapsis in desynaptic mutants sy 7 and sy 10. Theor Appl Genet. 1994;88:1029–36.
Gottschalk W, Klein HD. The influence of mutated genes in sporogenesis. A survey on the genetic control of meiosis in Pisum sativum. Theor Appl Genet. 1976;48:23–34.
Goyal S, Khan SA. Comparative study of chromosomal aberrations in Vigna mungo induced by ethyl methane sulphonate and hydrazine hydrate. Thail J Agric Sci. 2009;42:177–82.
Gulfishan M, Jafri IF, Khan AH, Bhat TA. Methyl methane sulphonate induced desynaptic mutants in Capsicum annuum L. Chromosom Bot. 2013;8:59–62.
Joshi P, Verma RC. Ethyl Methane Sulphonate (EMS) induced (Partial) Asynaptic mutant in Faba bean (Vicia faba L.). Cytologia. 2005;70:143–7.
Kaul MLH, Nirmala C. Male sterility in pea II. Male sex specific dys-synapsis. Cytologia. 1993;58:67–76.
Khawaja HII, Ellis JR. Colchicine-induced desynaptic mutations in Lathyrus odoratus L. and L. pratensis L. Genome. 1987;29:859–66.
Koduru PRK, Rao MK. Cytogenetics of synaptic mutants in higher plants. Theor Appl Genet. 1981;59:197–214.
Konvicka O, Gottschalk W. Untersuchngen an der meiosis steriler mutanten von Allium cepa. Angew Botanik. 1974;48:9–19.
LaCour LP, Wells B. Meiotic prophase in anthers of asynaptic Wheat. A light and electron microscopic study. Chromosoma. 1970;29:419–27.
Mahandjiev A, Kosturkova G, Mihov M. Enrichment of Pisum sativum gene resources through combined use of physical and chemical mutagens. Isr J Plant Sci. 2001;49:279–84.
Palmer RG, Kaul MLH. Genetics, cytology and linkage studies of a desynaptic soybean mutant. J Hered. 1983;74:260–4.
Panda RC, Kumar OA, Rao KGR. Desynaptic mutant in chili pepper. J Hered. 1987;78:101–4.
Pena ADL, Puertas MC, Cermeno MC, Girraldez R. Evidence of crossing-over inhibition in Rye anthers culture with colchicine. Chromosoma. 1979;72:151–5.
Prakken R. Studies of asynapsis in Rye. Hereditas. 1943;29:475–95.
Ramakrisnan P. Biostatistics. Nagercoil: Saras Publications; 2005.
Sharma SK, Bisht MS, Pandit MK. Synaptic mutation-driven male sterility in Panax sikkimensis Ban. (Araliaceae) from Eastern Himalaya, India. Pl Syst Evol. 2010;287:29–36.
Singh MR. Cytogenetic studies of induced desynaptic mutants in Barley (Hordeum vulgare L.). Cytologia. 2002;67:129–33.
Singh RJ. Plant cytogenetics. 2nd ed. New York: CRC press Boca Raton London; 2003.
Sjodin J. Induced asynaptic mutants in Vicia faba L. Hereditas. 1970;66:215–32.
Stephens JC, Schertz KF. Asynapsis and its inheritance in Sorghum vulgare. Pers Crop Sci. 1965;5:337–9.
Verma RC, Ahirwar R. Chromosomal behavior in Rhoeo discolor against colchicine treatment. Cytologia. 2012;77:17–21.
Verma RC, Raina SN. Cytogenetics of Crotalaria VI. Chiasma frequency and position, and univalent behavior in a (Partially) asynaptic mutant of C. juncea. Genetica. 1982;58:65–70.
Verma RC, Vyas P, Raina SN. The effect of colchicine on meiosis in the Rhoeo discolor stabile complex translocation heterozygote. Genome. 1992;35:611–3.
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The authors thankfully acknowledge to Madhya Pradesh Council of Science and Technology, Bhopal for financial support.
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Ahirwar, R., Verma, R.C. Colchicine induced asynaptic chromosomal behavior at meiosis in Allium cepa L.. Nucleus 58, 47–51 (2015). https://doi.org/10.1007/s13237-015-0133-4
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DOI: https://doi.org/10.1007/s13237-015-0133-4