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Bone-regenerative activity of parathyroid hormone-releasing nano-hydroxyapatite/poly(L-lactic acid) hybrid scaffolds

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Abstract

We developed a bone-regenerative scaffold based on systematic combination of porous organic-inorganic hybrid scaffolds and recombinant human parathyroid hormone (rhPTH). The hybrid scaffold was fabricated by immobilization of polyphosphate-functionalized nano-hydroxyapatite (PP-n-HAp) on the surface of porous poly(L-lactic acid) (PLLA) scaffolds, which was followed by rhPTH loading on the polyphosphates of n-HAp surfaces. The surface polyphosphate functionalities of PP-n-HAp enabled the stable chemical immobilization of n-HAp on the amine-treated pore surface of the PLGA scaffolds. rhPTH with a positive charge was bound at a high efficiency of 98.1~99.5% onto the anionic polyphosphates of PP-n-HAp immobilized on PLLA surfaces and was sustainably released for up to 50 days. The release rate was manipulated by adjusting the amount of loaded rhPTH, and the release data were moderately fitted to the Higuchi’s diffusion model. Four types of scaffolds were tested in rabbit calvarias models (PLLA only, PP-n-HAp-PLLA, rhPTH (2 µg) loaded PP-n-HAp-PLLA, and rhPTH (10 µg) loaded PP-n-HAp-PLLA). After 5 weeks, rhPTH-loaded PP-n-HAp-PLLA (2 and 10 µg of rhPTH) displayed higher bone growth than the control (PLLA only) group. Nano-HAp and sustained release of rhPTH might be synergistically able to enhance the bone healing in the animal model.

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Authors and Affiliations

  1. Department of Maxillofacial Biomedical Engineering & Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 02447, Korea

    Ahn Na Koo, Hong Jae Lee & Sang Cheon Lee

  2. Department of Oral & Maxillofacial Surgery, School of Dentistry, Kyung Hee University, Seoul, 02447, Korea

    Joo-Young Ohe, Deok-Won Lee, Jeewan Chun & Yong-Dae Kwon

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  1. Ahn Na Koo
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  2. Joo-Young Ohe
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  3. Deok-Won Lee
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  4. Jeewan Chun
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  5. Hong Jae Lee
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  6. Yong-Dae Kwon
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  7. Sang Cheon Lee
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Correspondence to Yong-Dae Kwon or Sang Cheon Lee.

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Koo, A.N., Ohe, JY., Lee, DW. et al. Bone-regenerative activity of parathyroid hormone-releasing nano-hydroxyapatite/poly(L-lactic acid) hybrid scaffolds. Macromol. Res. 23, 1168–1173 (2015). https://doi.org/10.1007/s13233-015-3157-3

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  • DOI: https://doi.org/10.1007/s13233-015-3157-3

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