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Can leaf degrading enzymes provide evidence that endophytic fungi becoming saprobes?

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Abstract

The diversity of endophytic and saprobic fungi from Magnolia liliifera leaves were observed and analyzed to establish relationships. Nine endophytes were morphologically and phylogenetically similar to the saprobes; Colletotrichum gloeosporioides, Colletotrichum sp. 2, Corynespora cassiicola, Fusarium sp. 1, Guignardia mangiferae, Leptosphaeria sp., Phomopsis sp. 2, Phomopsis sp. 6, and Phomopsis sp. 10. The endophytes were found to produce the same degrading enzymes as their saprobic counterparts. The isoform of β-mannanase produced from each of endophyte and saprobe counterparts were similar. Fungal succession and enzyme production patterns during leaf decomposition were correlated. The occurrence of saprobes was found to be related to the enzymes that the fungi produce. The study provides further compelling evidence that endophytes can switch lifestyle to saprobes.

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Acknowledgements

We would like to thank The Thailand Research Fund [The Royal Golden Jubilee Ph.D. Program (4.B.CM/44/D.1)] for the award of Postgraduate Studentship 2001–2006 to I. Promputtha. Thank for partial support from Conservation and Utilization of Biodiversity Resources Program, Faculty of Science, Chiang Mai University. We are also grateful to Dr. Chartchai Khanongnuch of the Department of Biotechnology, Faculty of Agro-Industry, Chiang Mai University and Mrs. Nittaya Boontim for their guidance and suggestion during the enzymatic study. Thanks for our entire colleagues and administrative staffs for their help and encouragement.

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Authors and Affiliations

  1. School of Cosmetic Science, Mae Fah Luang University, 333 Moo 1, Tasud, Muang, Chiang Rai, 57100, Thailand

    Itthayakorn Promputtha

  2. School of Science, Mae Fah Luang University, 333 Moo 1, Tasud, Muang, Chiang Rai, 57100, Thailand

    Kevin D. Hyde

  3. Landcare Research, Private Bag 92170, Auckland, New Zealand

    Eric H. C. McKenzie

  4. University of Nottingham Institute for Enterprise and Innovation, Nottingham University Business School, Jubilee Campus, Nottingham, NG8 1BB, UK

    John F. Peberdy

  5. Faculty of Science, Department of Biology, Chiang Mai University, Chiang Mai, 50200, Thailand

    Saisamorn Lumyong

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  1. Itthayakorn Promputtha
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  2. Kevin D. Hyde
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Correspondence to Itthayakorn Promputtha.

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Promputtha, I., Hyde, K.D., McKenzie, E.H.C. et al. Can leaf degrading enzymes provide evidence that endophytic fungi becoming saprobes?. Fungal Diversity 41, 89–99 (2010). https://doi.org/10.1007/s13225-010-0024-6

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