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PAX1 Methylation Status in Cervical Scrapes as Novel Diagnostic Biomarker in CIN 2/3 and Invasive Squamous Cell Carcinoma

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Abstracts

Objectives

DNA methylation of paired box-1 (PAX-1) gene has been shown to be a potential biomarker for the detection of high-grade cervical intra-epithelial neoplasia (CIN) and invasive cervical cancer. The objective of this pilot study was to quantify and compare methylation percentage of PAX1 gene in benign cervical lesion, pre-invasive and invasive cervical cancer.

Methods

A total of 200 screen positive women (VIA, VILI and Pap test) underwent colposcopy. Cervical scrapes taken were taken and stored for DNA analysis and PAX 1 methylation status. Women with Swede score of 5 or more (n = 98) were biopsied. Cervical scrapes and biopsy were taken from women with obvious cervical growth (n = 14), without prior colposcopy. Sixty women were recruited to the study and allocated into three groups on the basis of histopathology, i.e., benign cervix (Group 1; n = 20), CIN 2/3 (Group 2; n = 20) and invasive cervical carcinoma (Group; n = 20). PAX 1 methylation percentage was calculated from the DNA extracted from the cervical scrapes of the women recruited.

Results

The mean PAX1 methylation percentage in benign lesions, CIN 2/3 and invasive cancer was 9.58% (SD ± 2.37%), 18.21% (SD ± 2.67%) and 24.34% (SD ± 4.09%), respectively, with p-value of < 0.001.

Conclusions

PAX 1 gene methylation has a promising role in identifying high-grade lesions and invasive cancer.

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Acknowledgements

This study was funded by Indian Council of Medical Research

Funding

The study was funded by Indian Council of Medical Research.

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Authors and Affiliations

  1. Department of Obstetrics & Gynaecology, UCMS & GTB Hospital, A-318, Mangal Apartments, Vasundhara Enclave, Delhi, India

    Bhavya, Bindiya Gupta & Sandhya Jain

  2. Division of Gynecologic Oncology, AIIMS, Rishikesh, India

    Shalini Rajaram

  3. Department of Biochemistry, UCMS & GTB Hospital, Delhi, India

    B. D. Banerjee & Gaurav Thakur

  4. Department of Pathology, UCMS & GTB Hospital, Delhi, India

    Vinod Kumar Arora

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  1. Bhavya
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  2. Shalini Rajaram
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Correspondence to Bhavya.

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No conflict of interest among the authors.

Ethical approval

Study has been approved by institutional ethics board and all procedures performed in study involving human participants were in accordance with the ethical standards of the institution and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all the participants included in the study.

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The work described has not been published before and is not under consideration for publication anywhere else. The publication has been approved by all co-authors at the institute where the work has been carried out.

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Dr Bhavya (MS) is a Senior Resident, Department of Obstetrics & Gynecology at UCMS & GTB hospital, Delhi, India; Shalini Rajaram (MD) is a Professor, Division of Gynecologic Oncology, AIIMS, Rishikesh, India; Bindiya Gupta (MD) is an Associate Professor, Department of Obstetrics & Gynecology at UCMS & GTB hospital, Delhi, India; B.D. Banerjee (MD) is a Professor, Department of Biochemistry, UCMS & GTB Hospital, Delhi, India; Vinod Kumar Arora (MD) is a Director Professor, Department of Pathology, UCMS & GTB Hospital, Delhi, India; Gaurav Thakur (Msc) is a Ph.D. Scholar, Department of Biochemistry, UCMS & GTB Hospital, Delhi, India; Sandhya Jain (MD) is an Associate Professor, Department of Obstetrics & Gynecology at UCMS & GTB hospital, Delhi, India.

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Bhavya, Rajaram, S., Gupta, B. et al. PAX1 Methylation Status in Cervical Scrapes as Novel Diagnostic Biomarker in CIN 2/3 and Invasive Squamous Cell Carcinoma. J Obstet Gynecol India 72, 522–528 (2022). https://doi.org/10.1007/s13224-022-01680-5

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  • DOI: https://doi.org/10.1007/s13224-022-01680-5

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