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Identification and phylogenetic relationship of Mannheimia varigena using the 16S rRNA subunit and the rpoB gene

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Abstract

The objective of this work was to identify Mannheimia varigena strains in samples of bovine nasal exudates and bovine pneumonic lung using the API 20E bacterial identification system, 16S rRNA gene sequencing, and rpoB gene sequencing, as well as the establishment of the phylogenetic relationship with the Pasteurellaceae family. The API 20E identification system determined the strains correspond to the genus Mannheimia spp. Sequencing of the 16S rRNA gene showed 100 % homology with Mannheimia varigena and sequencing of the rpoB gene showed 99 % homology with M. varigena; phylogenetic analysis confirmed the isolates belong to M. varigena by locating the isolates in a separate and independent clade. In contrast to sequences from bovine pneumonic lung, the amino acid sequence of the rpoB gene from bovine nasal exudates showed two different mutations, the changing of aspartate for asparagine at position 158 and alanine for aspartate at position 160. These mutations are non-synonymous changes that have not been reported in the Mannheimia complex or related species.

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Acknowledgments

This study was financed by PAPIIT-UNAM grant-IN208708 and CONACYT CB104031.

Ethical standards

All animal manipulations and procedures were approved by the Internal Committee for Laboratory Animal Welfare of the National Autonomous University of Mexico following ethical international standards.

Conflict of interest

The authors have no conflicts of interest.

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Authors and Affiliations

  1. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, 04510, Mexico

    Zaydy Suástegui-Urquijo, Carlos Julio Jaramillo-Arango, Francisco Trigo-Tavera & Francisco Suárez-Güemes

  2. Centro de Enseñanza, Investigación y Extensión en Producción Animal en Altiplano, Km 8.5 Tequisquiapan-Ezequiel Montes, 76750, Querétaro, Mexico

    Carlos Julio Jaramillo-Arango

  3. Departamento de Ecología de Agentes Patógenos, Dirección de Investigación, Hospital General “Dr. Manuel Gea González”, Tlálpan, 14080, Mexico

    Fernando Martínez-Hernández, Elizabeth Ureta & Rigoberto Hernández-Castro

  4. Laboratorio de Bacteriología Intestinal, Departamento de Infectología, Hospital Infantil de México Federico Gómez, Ciudad de México, Mexico

    Juan Xicohtencatl-Cortes

Authors
  1. Zaydy Suástegui-Urquijo
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  2. Carlos Julio Jaramillo-Arango
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  3. Fernando Martínez-Hernández
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  4. Elizabeth Ureta
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  5. Francisco Trigo-Tavera
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  6. Francisco Suárez-Güemes
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  7. Juan Xicohtencatl-Cortes
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  8. Rigoberto Hernández-Castro
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Corresponding author

Correspondence to Rigoberto Hernández-Castro.

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Suástegui-Urquijo, Z., Jaramillo-Arango, C.J., Martínez-Hernández, F. et al. Identification and phylogenetic relationship of Mannheimia varigena using the 16S rRNA subunit and the rpoB gene. Ann Microbiol 65, 1781–1787 (2015). https://doi.org/10.1007/s13213-014-1017-6

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  • DOI: https://doi.org/10.1007/s13213-014-1017-6

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