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Improved stability and reusability of cotton-immobilized recombinant Escherichia coli producing US132 Cyclodextrin Glucanotransferase

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Abstract

Recombinant Escherichia coli / pAD26 cells were optimally immobilized on cotton to obtain plasmid stability. An experimental design that monitored two major responses, namely stability and reusability, was applied for screening the factors affecting the immobilization of the strain (cylodextrin, sucrose, shape support, mass of cotton matrices, mass of initial biomass, age of biomass, ethylenediamine, glutaraldehyde, and incubation time) and the optimization of the most significant variables. The optimal values recorded for the two criteria were obtained with high glutaraldehyde concentration (0.15 %) and early inoculum age (2 h) in the presence of 0.2 mM cylodextrin. With those optimum conditions, cyclodextrin glucanotransferase production yields were enhanced, reaching up to 39.77 U/mL. The stability and reusability of the immobilized strain were two fold and three fold higher, respectively, than those of the control after three cycles.

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Acknowledgments

This work was funded by the Tunisian Ministry of Higher Education and Scientific Research and Technology (contract program LMB-CBS, grant no. RL02CBS01). The authors would like to express their sincere gratitude to Mr. Anouar Smaoui and Mrs. Hanen Ben Salem from the English Language Unit at the Faculty of Science of Sfax for their constructive language editing and proofreading services.

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Authors and Affiliations

  1. Laboratory of Microrganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road Sidi Mansour Km 6, B.P 1177, Sfax, 3018, Tunisia

    Mouna Kriaa, Dorra Ayadi-Zouari, Mouna Sahnoun, Sonia Jemli, Samir Bejar & Radhouane Kammoun

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  1. Mouna Kriaa
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  2. Dorra Ayadi-Zouari
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  3. Mouna Sahnoun
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  4. Sonia Jemli
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  5. Samir Bejar
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  6. Radhouane Kammoun
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Correspondence to Radhouane Kammoun.

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Kriaa, M., Ayadi-Zouari, D., Sahnoun, M. et al. Improved stability and reusability of cotton-immobilized recombinant Escherichia coli producing US132 Cyclodextrin Glucanotransferase. Ann Microbiol 65, 383–391 (2015). https://doi.org/10.1007/s13213-014-0870-7

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  • DOI: https://doi.org/10.1007/s13213-014-0870-7

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