Abstract
16S rRNA, rpoB, and sodA genes of Mycobacterium celatum type 2 were re-examined to clarify its taxonomic position. Reevaluation of these sequences revealed that M. celatum type 2 strain ATCC 51130 was 100% identical to Mycobacterium kyorinense type strain, but differed substantially from M. celatum types 1 and 3 at these genes. A phylogenetic tree analysis placed M. celatum type 2 in proximity with M. kyorinense, with a clear-cut distinction from M. celatum types 1 and 3. We propose the reclassification of M. celatum type 2 as M. kyorinense, with both species maintaining their respective names and standing in the literature.
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References
Bull TJ, Shanson DC, Archard LC, Yates MD, Hamid ME, Minnikin DE (1995) A new group (Type 3) of Mycobacterium celatum isolated from AIDS patients in the London area. Int J Syst Bacteriol 45:861–862
Butler WR, O'Connor SP, Yakrus MA, Smithwick RW, Plikaytis BB, Moss CW, Floyd MM, Woodley CL, Kilburn JO, Vadney FS, Gross WM (1993) Mycobacterium celatum sp. nov. Int J Syst Bacteriol 43:539–548
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
Gholizadeh Y, Varnerot A, Maslo C, Salauze B, Badaoui H, Vincent V, Buré-Rossier A (1998) Mycobacterium celatum infection in two HIV-infected patients treated prophylactically with rifabutin. Eur J Clin Microbiol Infect Dis 17:278–281
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Koukila-Kahkola P, Springer B, Bottger EC, Paulin L, Jantzen E, Katila ML (1995) Mycobacterium branderi sp. nov., A new potential human pathogen. Int J Syst Bacteriol 45:549–553
Ohnishi H, Yonetani S, Matsushima S, Wada H, Takeshita K, Kuramochi D, Caldas PC, Campos CE, Da Costa BP, Ramos JP, Mikura S, Narisawa E, Fujita A, Funayama Y, Kobashi Y, Sakakibara Y, Ishiyama Y, Takakura S, Goto H, Watanabe T (2013) Mycobacterium kyorinense infection. Emerg Infect Dis 19:508–510
Okazaki M, Ohkusu K, Hata H, Ohnishi H, Sugahara K, Kawamura C, Fujiwara N, Matsumoto S, Nishiuchi Y, Toyoda K, Saito H, Yonetani S, Fukugawa Y, Yamamoto M, Wada H, Sejimo A, Ebina A, Goto H, Ezaki T, Watanabe T (2009) Mycobacterium kyorinense sp. Nov., A novel, slow-growing species, related to Mycobacterium celatum, isolated from human clinical specimens. Int J Syst Evol Microbiol 59:1336–1341
Picardeau M, Bull TJ, Prod'hom G, Pozniak AL, Shanson DC, Vincent V (1997) Comparison of A new insertion element, IS1407, with established molecular markers for the characterization of Mycobacterium celatum. Int J Syst Bacteriol 47:640–644
Piersimoni C, Tortoli E, De Lalla F, Nista D, Donato D, Bornigia S, De Sio G (1997) Isolation of Mycobacterium celatum from patients infected with human immunodeficiency virus. Clin Infect Dis 24:144–147
Ramos JP, Campos CE, Caldas PC, Ferreira NV, Da Silva MV, Redner P, Campelo CL, Vale SF, Barroso EC, Medeiros RF, Montes FC, Galvão TC, Tortoli E (2013) Mycobacterium fragae sp. nov., A non-chromogenic species isolated from human respiratory specimens. Int J Syst Evol Microbiol 63:2583–2587
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Springer B, Stockman L, Teschner K, Roberts GD, Böttger EC (1996) Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34:296–303
Stadthagen-Gomez G, Helguera-Repetto AC, Cerna-Cortes JF, Goldstein RA, Cox RA, Gonzalez-Y-Merchand JA (2008) The organization of two Rrna (Rrn) operons of the slow-growing pathogen Mycobacterium celatum provides key insights into mycobacterial evolution. FEMS Microbiol Lett 280:102–112
Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P, Maiden MC, Nesme X, Rosselló-Mora R, Swings J, Trüper HG, Vauterin L, Ward AC, Whitman WB (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Tortoli E, Piersimoni C, Bacosi D, Bartoloni A, Betti F, Bono L, Burrini C, De Sio G, Lacchini C, Mantella A, Orsi PG, Penati V, Simonetti MT, Bottger EC (1995) Isolation of the newly described species Mycobacterium celatum from AIDS patients. J Clin Microbiol 33:137–140
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This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (23590688).
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Ohnishi, H., Tortoli, E., Ramos, J.P. et al. Proposal of reclassification of Mycobacterium celatum type 2 as Mycobacterium kyorinense . Ann Microbiol 64, 1879–1882 (2014). https://doi.org/10.1007/s13213-014-0860-9
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DOI: https://doi.org/10.1007/s13213-014-0860-9