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Identification and nematicidal activity of bacteria isolated from cow dung

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Abstract

Two hundred and nineteen bacterial strains were isolated from cow dung. Among these, 59 isolates displayed nematicidal activity against the model nematode Caenorhabditis elegans. Of the 59 bacterial strains, 17 killed >90 % of the tested nematodes within 1 h. Based on their 16S rRNA sequences, these 17 strains were identified as Alcaligenes faecalis, Bacillus cereus, Proteus penneri, Providencia rettgeri, Pseudomonas aeruginosa, Pseudomonas otitidis, Staphylococcus sciuri, Staphylococcus xylosus, Microbacterium aerolatum, Pseudomonas beteli. Among these 17 strains, 14 produced volatile organic compound(s) that inhibited the mobility of the C. elegans nematodes. These 14 strains also showed nematicidal activity against a plant pathogenic nematode Meloidogyne incognita. This is the first report demonstrating nematicidal activity for strains in genera Proteus, Providencia and Staphylococcus.

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Acknowledgments

The work was supported by grants from the “973” Program of China (2013CB127505, 2012CB722208) and the NSFC (30960007), the Young Academic and Technical Leader Raising Foundation of Yunnan Province (2010CI023), and the Program from Yunnan Provincial Company (2010yn17) and the China National Tobacco Corporation (110201002023).

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Authors and Affiliations

  1. Key Laboratory for Conservation and Utilization of Bio-resource, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, Yunnan, China

    Hao Lu, Xin Wang, Keqin Zhang, Youyao Xu, Liang Zhou & Guohong Li

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  1. Hao Lu
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  2. Xin Wang
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  3. Keqin Zhang
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  4. Youyao Xu
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  5. Liang Zhou
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  6. Guohong Li
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Correspondence to Guohong Li.

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Lu, H., Wang, X., Zhang, K. et al. Identification and nematicidal activity of bacteria isolated from cow dung. Ann Microbiol 64, 407–411 (2014). https://doi.org/10.1007/s13213-013-0660-7

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