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A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae

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Abstract

To find potential biocontrol agents for Plasmodiophora brassicae, a total of 63 actinobacteria were isolated from the rhizospheric soil of Chinese cabbage that were collected from Sichuan province in China. Isolates were screened by measuring the resting spores' germination inhibition. The suppressive effects of six antagonistic strains against clubroot on Chinese cabbage in the glasshouse and the field were assessed, respectively. Strain A316 was the most promising antagonist against clubroot, which showed the highest control values of 73.69% in the glasshouse experiment and 65.91% in the field trial. Based on the 16S rDNA sequence analysis, strain A316 was most closely related to Streptomyces griseoruber NBRC 12873 (99.9% similarity). Together with morphological, cultural and physio-biochemical characteristics, strain A316 was identified as Streptomyces griseoruber. The results indicate that the isolate A316 might have the potential possibility to be used as an antagonist against P. brassicae. This is the first report of S. griseoruber for controlling clubroot.

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Acknowledgement

This work was supported by grant from the Commonweal Specialized Research Fund of China Agriculture (201003029).

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  1. Department of Plant Pathology, Sichuan Agricultural University, No.555 Dongbei Road of Wenjiang district, Chengdu, 611130, China

    Jing Wang, Yun Huang, Shan Lin, Fan Liu, Qin Song, Yulong Peng & Liu Zhao

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  1. Jing Wang
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  2. Yun Huang
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  3. Shan Lin
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  4. Fan Liu
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  5. Qin Song
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  6. Yulong Peng
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  7. Liu Zhao
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Correspondence to Yun Huang.

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Wang, J., Huang, Y., Lin, S. et al. A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae . Ann Microbiol 62, 247–253 (2012). https://doi.org/10.1007/s13213-011-0253-2

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  • DOI: https://doi.org/10.1007/s13213-011-0253-2

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