Abstract
Considering the factor of culture, religion, health and commerce, the development of a rapid, convenient and sensitive method is important for the detection of the presence of meat species in raw or processed foods. In this study, we employed loop mediated isothermal amplification (LAMP) for species-specific target gene amplification, and the amplicons were analyzed on the immunochromatographic strip (ICS) for the rapid visual identification of meat species. Bovine meat was selected as an example to confirm the performance of such assay. The LAMP based ICS method show good specificity, repeatability and has a limit of detection of 0.1% for beef in meat mixture. The whole detection process could be completed within 50 min. Our method is more convenient and rapid compared to classical LAMP. Moreover, it could be easily adapted to identify other types of meats, and it may be useful for food administration laboratories to carry out meat species identification in raw and processed foods.
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References
Macedo-Silva, A. et al. Hamburger meat identification by dot-ELISA. Meat Science 56, 189–192 (2000).
Asensio, L., González, I., García, T. & Martín, R. Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA). Food Control 19, 1–8 (2008).
Koh, M. et al. Random amplified polymorphic DNA (RAPD) fingerprints for identification of red meat animal species. Meat Science 48, 275–285 (1998).
Matsunaga, T. et al. A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Science 51, 143–148 (1999).
Sasazaki, S. et al. Development of breed identification markers derived from AFLP in beef cattle. Meat Science 67, 275–280 (2004).
Girish, P. et al. Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene. Meat Science 70, 107–112 (2005).
Ghovvati, S. et al. Fraud identification in industrial meat products by multiplex PCR assay. Food Control 20, 696–699 (2009).
Rashid, P.M.A., Babashekh, M.O., Marouf, A.S. & Amin, K.M. Identification of Animal Species in Meat Broth by Simplex and Multiplex PCR. J. Zankoy Sulaimani-Part A (JZS-A) 16, 97–102 (2014).
Kesmen, Z., Gulluce, A., Sahin, F. & Yetim, H. Identification of meat species by TaqMan-based real-time PCR assay. Meat Science 82, 444–449 (2009).
Kesmen, Z., Yetiman, A.E., Sahin, F. & Yetim, H. Detection of chicken and turkey meat in meat mixtures by using Real-Time PCR Assays. J. Food Sci. 77, C167–C173 (2012).
Oellingrath, I.M., Iversen, A. & Skrede, G. Quantitative determination of myoglobin and haemoglobin in beef by high-performance liquid chromatography. Meat Science 28, 313–320 (1990).
von Bargen, C. et al. New Sensitive High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Detection of Horse and Pork in Halal Beef. J. Agric. Food Chem. 61, 11986–11994 (2013).
Mamani-Linares, L., Gallo, C. & Alomar, D. Identification of cattle, llama and horse meat by near infrared reflectance or transflectance spectroscopy. Meat Science 90, 378–385 (2012).
Haye, P.A. et al. Authentication of commercialized crab-meat in Chile using DNA barcoding. Food Control 25, 239–244 (2012).
Vaagt, F., Haase, I. & Fischer, M. Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification. J. Agric. Food Chem. 61, 1833–1840 (2013).
Ahmed, M.U. et al. Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor. Food Control 21, 599–605 (2010).
Yang, L. et al. Identification of pork in meat products using real-time loop-mediated isothermal amplification. Biotechnol. Biotechnol. Equip. 28, 882–888 (2014).
Saull, J., Duggan, C., Hobbs, G. & Edwards, T. The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process. Food Control 59, 306–313 (2016).
Notomi, T., Mori, Y., Tomita, N. & Kanda, H. Loopmediated isothermal amplification (LAMP): principle, features, and future prospects. J. Microbiol. 53, 1–5 (2015).
Mori, Y., Kitao, M., Tomita, N. & Notomi, T. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J. Biochem. Biophys. Methods 59, 145–157 (2004).
Tomita, N., Mori, Y., Kanda, H. & Notomi, T. Loopmediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Protoc. 3, 877–882 (2008).
Jung, J.H. et al. Combination of multiplex reverse-transcription loop-mediated isothermal amplification with an immunochromatographic strip for subtyping influenza A virus. Anal. Chim. Acta 853, 541–547 (2015).
Gao, H. et al. Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol. Anal. Chim. Acta 839, 91–96 (2014).
Yetisen, A.K., Akram, M.S. & Lowe, C.R. Paper-based microfluidic point-of-care diagnostic devices. Lab Chip 13, 2210–2251 (2013).
Peter, C., Brünen-Nieweler, C., Cammann, K. & Börchers, T. Differentiation of animal species in food by oligonucleotide microarray hybridization. Eur. Food Res. Technol. 219, 286–293 (2004).
Savan, R., Kono, T., Itami, T. & Sakai, M. Loop-mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens. J. Fish Dis. 28, 573–581 (2005).
Abdulmawjood, A. et al. Development of loop-mediated isothermal amplification (LAMP) assay for rapid Copyright for the published papers belongs to the Korean BioChip Society. pISSN 1976-0280. eISSN 2092-7843 and sensitive identification of ostrich meat. PLoS ONE 9, e100717 (2014).
Inácio, J., Flores, O. & Spencer-Martins, I. Efficient identification of clinically relevant Candida yeast species by use of an assay combining panfungal loop-mediated isothermal DNA amplification with hybridization to species-specific oligonucleotide probes. J. Clin. Microbiol. 46, 713–720 (2008).
Thekisoe, O.M. et al. Stability of loop-mediated isothermal amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates. J. Vet. Med. Sci. 71, 471–475 (2009).
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Li, YJ., Fan, JY. Rapid visual identification of bovine meat by loop mediated isothermal amplification combined with immunochromatographic strip. BioChip J 11, 8–13 (2017). https://doi.org/10.1007/s13206-016-1102-y
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DOI: https://doi.org/10.1007/s13206-016-1102-y