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Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray

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Abstract

Human Leukocyte Antigen (HLA), called human Major Histocompatibility Complex (MHC), performs the crucial function of antigen presentation in human immunity, and correctly matched HLA antigens improves survival and reduces immunological complications. Therefore, a precise HLA typing method is necessary for successful transplantation between donor and recipient. Until now, many methods have been developed for HLA typing, including serological and DNA-based methods. However, these previous methods have been somewhat limited due to their requirements for a large number of samples and complex experimental procedures. To resolve this problem, in this study, we developed rapid genotyping methods for HLA-C using an oligonucleotide microarray. To evaluate the performance of the HLA-C genotyping microarray, we analyzed 50 unrelated Korean individuals. The genotyping results obtained by the oligonucleotide microarray were identical with those of PCR-SBT and PCR-SSPs, at a low-resolution, 2-digit level. Therefore, this microarray method for genotyping HLA constitutes a rapid, simultaneous, and high-throughput method that can be used as an alternative to current methods.

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Authors and Affiliations

  1. Department of Biochemistry, Hanyang University, Sa-dong, Sangnok-gu, Ansan, Gyeonggi-do, 426-791, Korea

    Hyun Kyu Yoon, In Hyuk Chung & Seung Yong Hwang

  2. GenoCheck Co., LTD., Sa-dong, Sangnok-gu, Ansan, Gyeonggi-do, 426-791, Korea

    Jun Sub Kim & Seung Yong Hwang

  3. Department of Laboratory Medicine & Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135-710, Korea

    Hee-Jin Kim

  4. Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, 135-710, Korea

    Ki-O Lee

  5. Bio-Med Connect Center, Samsung Medical Center, Sungkyunkwan University, Seoul, 136-710, Korea

    Eui-Jin Park, Sang Yong Song & Sa Ik Bang

  6. Department of Pathology, Samsung Medical Center, Sungkyunkwan University, Seoul, 136-710, Korea

    Sang Yong Song

  7. Department of Plastic Surgery, Samsung Medical Center, Sungkyunkwan University, Seoul, 136-710, Korea

    Sa Ik Bang

  8. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Tae Sung Kim

Authors
  1. Hyun Kyu Yoon
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  2. In Hyuk Chung
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Correspondence to Tae Sung Kim or Seung Yong Hwang.

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Yoon, H.K., Chung, I.H., Kim, HJ. et al. Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray. BioChip J 5, 255–264 (2011). https://doi.org/10.1007/s13206-011-5310-1

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  • DOI: https://doi.org/10.1007/s13206-011-5310-1

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