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Graphene-based electrochemical biosensor for pathogenic virus detection

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Abstract

A graphene based biosensor system is presented for performing highly sensitive pathogenic virus detection. A free-standing conductive graphene film was prepared as a novel electrochemical sensor through two steps: synthesis of a graphene oxide (GO) film from GO colloidal suspensions by using a speed vacuum concentrator and thermal annealing process at 900°C with H2/Ar flow to generate a reduced GO film. The resultant graphene film shows an excellent electron transfer property on the surface in the [Fe(CN)6]3−/4− redox system and is used as a working electrode in the electrochemical biosensor. The surface of graphene is modified with pyrene derivatives, and then covalently linked with virus-specific antibodies. The target cell, rotavirus, is captured on the graphene film through antibody-antigen interaction, and the entire process was monitored by cyclic voltammetric responses. A 105 pfu/mL of input cells is detected with ca. 30.7% sensitivity, and ca. 1.3% sensitivity is measured with 103 pfu/mL of input cells, demonstrating that graphene film based electrode can be applied for electrochemical biosensor.

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Authors and Affiliations

  1. Integrative NanoBioMEMS Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Korea

    Fei Liu, Ki Seok Choi & Tae Seok Seo

  2. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Korea

    Tae Jung Park & Sang Yup Lee

Authors
  1. Fei Liu
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  2. Ki Seok Choi
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  3. Tae Jung Park
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  4. Sang Yup Lee
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  5. Tae Seok Seo
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Correspondence to Tae Seok Seo.

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Liu, F., Choi, K.S., Park, T.J. et al. Graphene-based electrochemical biosensor for pathogenic virus detection. BioChip J 5, 123–128 (2011). https://doi.org/10.1007/s13206-011-5204-2

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  • DOI: https://doi.org/10.1007/s13206-011-5204-2

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