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IAA-decorated CuO nanocarriers significantly improve Chickpea growth by increasing antioxidative activities

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Abstract

Plant growth regulators tagged on metallic oxide nanoparticles (NPs) may function as nanofertilizers with reduced toxicity of NPs. CuO NPs were synthesized to function as nanocarriers of Indole-3-acetic acid (IAA). Powder X-ray diffraction (XRD) and scanning electron microscopy (SEM) revealed 30.4 nm size of NPs and sheet-like structure, respectively, of CuO-IAA NPs. Fourier-transform infrared spectroscopy (FTIR) confirmed CuO-IAA formation. IAA-decorated CuO NPs enhanced the physiological parameters of Chickpea plants, i.e., root length, shoot length, and biomass compared to naked CuO NPs. The variation in physiological response was due to change of phytochemical contents in plants. Phenolic content increased up to 17.98 and 18.13 µgGAE/mg DW at 20 and 40 mg/L of CuO-IAA NPs, respectively. However, significant decrease in antioxidant enzymes’ activity was recorded compared to control. Presence of CuO-IAA NPs increased the reducing potential of plants at higher concentration of NPs, while decrease in total antioxidant response was observed. This study concludes that IAA conjugation to CuO NPs reduces toxicity of NPs. Furthermore, NPs can be explored as nanocarriers for plant modulators and slow release in future studies.

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Acknowledgements

Authors are thankful to the Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan for providing all research facilities.

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Authors and Affiliations

  1. Department of Biotechnology, Quaid-I-Azam University, Islamabad, 45320, Pakistan

    Saad Hanif, Aisha Khan, Anila Sajjad & Muhammad Zia

  2. School of Science and the Environment, Grenfell Campus, Memorial University of Newfoundland and Labrador, Corner Brook, NF, A2H 5G4, Canada

    Rabia Javed

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  1. Saad Hanif
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SH: writing—original draft, formal analysis, data curation, validation RJ: investigation, validation, writing—original draft, writing—review and editing, visualization AK: writing—original draft, data curation AS: writing—original draft MZ: conceptualization, methodology, resources, supervision, project administration.

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Hanif, S., Javed, R., Khan, A. et al. IAA-decorated CuO nanocarriers significantly improve Chickpea growth by increasing antioxidative activities. 3 Biotech 13, 104 (2023). https://doi.org/10.1007/s13205-023-03516-z

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