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Whole genome analysis of Gluconacetobacter azotocaptans DS1 and its beneficial effects on plant growth

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Abstract

Plant-associated bacteria play an important role in the enhancement of plant growth and productivity. Gluconacetobacter azotocaptans is an exceptional bacterium considering that till today it has been isolated and reported only from Mexico and Canada. It is a plant growth-promoting bacterium and can be used as biofertilizer for different crops and vegetables. The objective of the current study was to evaluate the inoculation effect of Gluconacetobacter azotocaptans DS1, Pseudomonas putida CQ179, Azosprillium zeae N7, Azosprillium brasilense N8, and Azosprillium canadense DS2, on the growth of vegetables including cucumber, sweet pepper, radish, and tomato. All strains increased the vegetables’ growth; however, G. azotocaptans DS1 showed better results as compared to other inoculated and control plants and significantly increased the plant biomass of all vegetables. Therefore, the whole genome sequence of G. azotocaptans DS1 was analyzed to predict genes involved in plant growth promotion, secondary metabolism, antibiotics resistance, and bioremediation of heavy metals. Results of genome analysis revealed that G. azotocaptans DS1 has a circular chromosome with a size of 4.3 Mbp and total 3898 protein-coding sequences. Based on functional analysis, genes for nitrogen fixation, phosphate solubilization, indole acetic acid, phenazine, siderophore production, antibiotic resistance, and bioremediation of heavy metals including copper, zinc, cobalt, and cadmium were identified. Collectively, our findings indicated that G. azotocaptans DS1 can be used as a biofertilizer and biocontrol agent for growth enhancement of different crops and vegetables.

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Acknowledgements

We are highly thankful to the Higher Education Commission, Pakistan (HEC; Project No. 20-3134) and Agriculture and Agri-Food Canada for funding of this research work. We also gratefully acknowledge Dr. Kamran Azeem (Mohammad Ali Jinnah University, Karachi, Pakistan) for his help in sequence analysis and bioinformatics work.

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Authors and Affiliations

  1. KAM School of Life Sciences, Forman Christian College (A Chartered University), Lahore, Pakistan

    Salma Mukhtar, Deeba Noreen Baig, Kauser Abdulla Malik & Samina Mehnaz

  2. Division of Agricultural Biotechnology, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, Faisalabad, Pakistan

    Muhammad Farooq & Imran Amin

  3. A & L Biologicals, Agroecology Research Services Centre, London, ON, N5V 3P5, Canada

    George Lazarovits

  4. Agriculture and Agri Food Canada, London, ON, Canada

    Ze-Chun Yuan

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  1. Salma Mukhtar
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  2. Muhammad Farooq
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Correspondence to Samina Mehnaz.

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Mukhtar, S., Farooq, M., Baig, D.N. et al. Whole genome analysis of Gluconacetobacter azotocaptans DS1 and its beneficial effects on plant growth. 3 Biotech 11, 450 (2021). https://doi.org/10.1007/s13205-021-02996-1

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