Abstract
Zelkova schneideriana Hand.-Mazz. of the Ulmaceae family is a Tertiary relict and economically deciduous tree species endemic to Central and Southern China. In this study, we performed a transcriptome sequencing of Z. schneideriana using high-throughput sequencing approach to detect polymorphic expressed sequence tag-simple sequence repeats (EST-SSR) markers. A total of 3,235 microsatellite loci were detected from 53,517 unigenes. A set of 30 microsatellite markers were randomly selected to validate in 41 individuals from three populations, of which 10 were polymorphic. The number of alleles per locus ranged from 3 to 11. The observed heterozygosity and expected heterozygosity ranged from 0.366 to 0.829 and 0.439 to 0.848, respectively. These polymorphic SSR primers showed good transferability across different Zelkova species, and are valuable for future studies on genetic diversity, conservation, phylogeography, and species delimitation in Z. schneideriana, as well as other Zelkova species.
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Data availability
All of the raw data for Zelkova schneideriana have been submitted to the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database under the accession number SRX9284372. The sequences including SSR motif were deposited to GenBank with accession number: MN852234–MN852243.
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Funding
This study was funded by the National Public Welfare Forestry Project (200904011), the National Natural Science Foundation of China (31600169), the Science Research and Technology Development Project of Guangxi (1598025-42) and The Open Project for Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation (15-B-03-01).
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LW, RZ, ML conceived and designed the research; LW, MG, YQ, HL collected the plant materials; LW, MG, LL, ML performed the research; LW, ML analysed the data; LW, RZ, ML wrote the paper. All authors have seen and approved the manuscript.
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Wang, L., Zhang, R., Geng, M. et al. De novo transcriptome assembly and EST-SSR markers development for Zelkova schneideriana Hand.-Mazz. (Ulmaceae). 3 Biotech 11, 420 (2021). https://doi.org/10.1007/s13205-021-02968-5
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DOI: https://doi.org/10.1007/s13205-021-02968-5