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Post-transcriptional suppression of G protein-coupled receptor 15 (GPR15) by microRNA-1225 inhibits proliferation, migration, and invasion of human colorectal cancer cells

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Abstract

The G protein-coupled receptors (GPRs) have been shown to regulate several cancer related processes. The aberrant expression of GPRs has been linked to the development of several cancers. The present study was designed to examine the expression and decipher the role of GPR15 in the development of human colorectal cancer. The results revealed GPR15 to be significantly (P < 0.05) upregulated in colorectal cancer cells. The silencing of GPR15 inhibited the growth of the colorectal cancer cells via induction of apoptosis. Induction of apoptosis in colorectal cancer cells was associated increase in Bax and decrease in Bcl-2 expression. The silencing of GPR-15 also caused a significant (P < 0.05) decline in the migration and invasion of the colorectal cancer cells. Bioinformatic analysis and luciferase assay revealed that the expression of GPR15 to be post-transcriptionally regulated by microRNA-1225 (miR-1225). The expression of miR-1225 was found to significantly (P < 0.05) downregulated in colorectal cancer cells and its overexpression caused suppression of GPR15 and inhibited the proliferation of the colorectal cancer cells. Nonetheless, overexpression of GPR15 could avoid the growth inhibitory effects of miR-1225. The results suggest that the GPR15/miR-1225 axis play an important role in the development of colon rectal cancer and exhibit therapeutic implications for its treatment.

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Acknowledgements

The authors acknowledge the experimental assistance from The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, China

Funding

This study was supported by Talent Project of Shanghai Pudong New Area Gongli Hospital (Grant NO: GLRq2-17–04).

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Authors and Affiliations

  1. Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, 215006, Suzhou, Jiangsu, People’s Republic of China

    Yuehui Guo & Caifang Ni

  2. Department of Interventioin, Gongli Hospital of Shanghai Pudong New Area, Shanghai, 200135, China

    Yuehui Guo, Qingyun Zhu, Shiwei Chen, Yanxiang Li, Daiquan Fu & Delin Qiao

Authors
  1. Yuehui Guo
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  2. Qingyun Zhu
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  3. Shiwei Chen
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  4. Yanxiang Li
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  5. Daiquan Fu
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  6. Delin Qiao
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  7. Caifang Ni
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Contributions

Conceptualization: YG, and CN; methodology: YG, QZ, CN. CS; formal analysis and investigation: YG, QZ, SC, YL, DF and DQ; writing—original draft preparation: YG, QZ and CN; writing—review and editing critically for important intellectual content: CN; supervision: CN.

Corresponding author

Correspondence to Caifang Ni.

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Conflict of interest

All the authors declare that he has no conflict of interest.

Ethical approval

A written informed consent was obtained from the patients before participation in the present study. The study was approved by the research ethics committee of the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of China under approval number AHSU-83/HUM-2019.

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Guo, Y., Zhu, Q., Chen, S. et al. Post-transcriptional suppression of G protein-coupled receptor 15 (GPR15) by microRNA-1225 inhibits proliferation, migration, and invasion of human colorectal cancer cells. 3 Biotech 11, 139 (2021). https://doi.org/10.1007/s13205-021-02682-2

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  • DOI: https://doi.org/10.1007/s13205-021-02682-2

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