Abstract
Tarballs are semi-solid blobs of crude-oil formed in marine environment. Microbial degradation of tarballs is poorly understood, though there are indications that tarball-associated microbes can degrade recalcitrant hydrocarbons present in tarballs. In this study, 38 tarball-associated bacteria from Betul beach, Goa, India were initially screened for crude oil degradation. Based on preliminary studies and literature survey, four bacterial strains, Alcanivorax sp. Betul-O, Marinobacter sp. Betul-26, Pseudomonas sp. Betul-14, and Pseudomonas sp. Betul-M were selected for bacterial consortia preparation. Eleven bacterial consortia were prepared and studied for degradation of n-alkanes and polycyclic aromatic hydrocarbon compounds (PAHs) of tarballs based on gravimetric and GC–MS–MS analyses. The bacterial consortia depleted 53.69–97.78% and 22.78–61.98% of n-alkanes and PAH compounds, respectively, within 45 days. Bacterial consortium comprising Pseudomonas sp. Betul-14, Pseudomonas sp. Betul-M, and Alcanivorax sp. Betul-O exhibited promising tarball degradation abilities with 97.78% and 61.98% degradation of n-alkanes and PAH, respectively, within 45 days. Further research is required to obtain insights into degradation products and possible pathways involved.
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References
Al-Sayegh A, Wahaibi Y, Joshi S, Al-Bahry S, Elshafie A, Al-Bemani A (2016) Bioremediation of heavy crude oil contamination. Open Biotechnol J 10:301–311
Bamforth SM, Singleton I (2005) Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions. J Chem Technol Biotechnol 80:723–736. https://doi.org/10.1002/jctb.1276
Barnes NM, Khodse VB, Lotlikar NP, Meena RM, Damare SR (2018) Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India. 3 Biotech 8:21. https://doi.org/10.1007/s13205-017-1043-8
Bidoia ED, Montagnolli RN, Lopes PRM (2010) Microbial biodegradation potential of hydrocarbons evaluated by colorimetric technique: a case study. In: Mendez-Vilas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology. Formatex Research Center, Badajoz, pp 1277–1288
Dellagnezze BM, De Sousa GV, Martins LL, Domingos DF, Limache EE, de Vasconcellos SP, da Cruz GF, de Oliveira VM (2014) Bioremediation potential of microorganisms derived from petroleum reservoirs. Mar Pollut Bull 89:191–200. https://doi.org/10.1016/j.marpolbul.2014.10.003
Fu B, Li QX, Xu T, Cui ZL, Sun Y, Li J (2014) Sphingobium sp. FB3 degrades a mixture of polycyclic aromatic hydrocarbons. Int Biodeterior Biodegrad 87:44–51. https://doi.org/10.1016/j.ibiod.2013.10.024
Hanson KG, Desai JD, Desai AJ (1993) A rapid and simple screening technique for potential crude oil degrading microorganisms. Biotechnol Tech 7:745–748. https://doi.org/10.1007/BF00152624
Hassanshahian M, Emtiazi G, Cappello S (2012) Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar Pollut Bull 64:7–12. https://doi.org/10.1016/j.marpolbul.2011.11.006
Hassanshahian M, Emtiazi G, Caruso G, Cappello S (2014) Bioremediation (bioaugmentation/biostimulation) trials of oil polluted seawater: a mesocosm simulation study. Mar Environ Res 95:28–38. https://doi.org/10.1016/j.marenvres.2013.12.010
Head IM, Jones DM, Roling WFM (2006) Marine microorganisms make a meal of oil. Nat Rev Microbiol 4:173–182. https://doi.org/10.1038/nrmicro1348
Itah AY, Essien JP (2005) Growth profile and hydrocarbonoclastic potential of microorganisms isolated from tarballs in the Bight of Bonny. Nigeria World J Microbiol Biotechnol 21:1317–1322. https://doi.org/10.1007/s11274-004-6694-z
Kanaly RA, Harayama S (2010) Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria. Microb Biotechnol 3:136–164. https://doi.org/10.1111/j.1751-7915.2009.00130.x
Kvenvolden KA, Cooper CK (2003) Natural seepage of crude oil into the marine environment. Geo-Mar Lett 23:140–146. https://doi.org/10.1007/s00367-003-0135-0
Liu C, Shao Z (2005) Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186. https://doi.org/10.1099/ijs.0.63443-0
Lotfinasabasl S, Gunale VR, Rajurkar NS (2012) Assessment of petroleum hydrocarbon degradation from soil and tarball by fungi. Biosci Discov 3:186–192
Mariano AP, Bonotto DM, Angelis DF, Pirôllo MPS, Contiero J (2008) Biodegradability of commercial and weathered diesel oils. Braz J Microbiol 39:133–142
Mishra S, Jeevan J, Kuhad RC, Lal B (2001) Evaluation of inoculum addition to stimulate in situ bioremediation of oily-sludge-contaminated soil. Appl Environ Microbiol 67:1675–1681. https://doi.org/10.1128/AEM.67.4.1675-1681.2001
Nair S, Lokabharathi PA (1977) Degradation of hydrocarbons by fungus, Fusarium sp. Ind J Mar Sci 6:173–175
Nkem BM, Halimoon N, Yusoff FM, Johari WLW, Zakaria MP, Reddy S, Kannan N (2016) Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach. Malays Mar Pollut Bull 107:261–268. https://doi.org/10.1016/j.marpolbul.2016.03.060
Peng RH, Xiong AS, Xue Y, Fu XY, Gao F, Zhao W, Tian YS, Yao QH (2008) Microbial biodegradation of polyaromatic hydrocarbons. FEMS Microbiol Rev 32:927–955. https://doi.org/10.1111/j.1574-6976.2008.00127.x
Perelo LW (2010) Review: in situ and bioremediation of organic pollutants in aquatic sediments. J Hazard Mater 177:81–89. https://doi.org/10.1016/j.jhazmat.2009.12
Randhwa M, Kaushal J (2014) Bioremediation through oilzapper and oilvorous-S technologies: a review. Eng Sci Int Res J 2:29–32
Santisi S, Cappello S, Catalfamo M, Mancini G, Hassanshahian M, Genovese L, Giuliano L, Yakimov M (2015) Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium. Braz J Microbiol 46:377–387. https://doi.org/10.1590/S1517-838246120131276
Seo JS, Keum YS, Li QX (2009) Bacterial degradation of aromatic compounds. Int J Environ Res Public Health 6:278–309. https://doi.org/10.3390/ijerph6010278
Shinde VL, Suneel V, Shenoy BD (2017) Diversity of bacteria and fungi associated with tarballs: recent developments and future prospects. Mar Pollut Bull 117:28–33. https://doi.org/10.1016/j.marpolbul.2017.01.067
Shinde VL, Meena RM, Shenoy BD (2018) Phylogenetic characterization of culturable bacteria and fungi associated with tarballs from Betul beach, Goa, India. Mar Pollut Bull 128:593–600. https://doi.org/10.1016/j.marpolbul.2018.01.064
Snellman EA, Collins RP, Cooke JC (1988) Utilization of fuel oils by fungi isolated from oceanic tarballs. Lett Appl Microbiol 6:105–107
Suneel V, Vethamony P, Naik BG, Krishna MS, Jadhav L (2015) Identifying the source of tar balls deposited along the beaches of Goa in 2013 and comparing with historical data collected along the West Coast of India. Sci Total Environ 527–528:313–321. https://doi.org/10.1016/j.scitotenv.2015.04.102
Tao Z, Bullard S, Arias C (2011) High numbers of Vibrio vulnificus in tarballs collected from oiled areas of the north-Central Gulf of Mexico following the 2010 BP Deep water horizon oil spill. EcoHealth 8:507–511. https://doi.org/10.1007/s10393-011-0720-z
Varjani SJ, Rana DP, Bateja S, Upasani VN (2013) Isolation and screening for hydrocarbon utilizing bacteria (HUB) from petroleum samples. Int J Curr Microbiol Appl Sci 2:48–60
Wang Z, Fingas M, Landriault M, Sigouin L, Castle B, Hostetter D, Zhang D, Spencer B (1998) Identification and linkage of tar balls from the coast of Vancouver Island and Northern California using GC/MS and Isotopic techniques. J High Resolut Chromatogr 21:383–395
Wang ZD, Fingas M, Page D (1999) Oil spill identification. J Chromatogr 843:369–411
Warnock AM, Hagen SC, Passeri DL (2015) Marine tar residues: a review. Water Air Soil Pollut 226:68. https://doi.org/10.1007/s11270-015-2298-5
Xue J, Yu Y, Bai Y, Wang L, Wu Y (2015) Marine oil-degrading microorganisms and biodegradation process of petroleum hydrocarbon in marine environments: a review. Curr Microbiol 71:220–228. https://doi.org/10.1007/s00284-015-0825-7
Yakimov MM, Timmis KN, Golyshin PN (2007) Obligate oil-degrading marine bacteria. Curr Opin Biotechnol 18:257–266. https://doi.org/10.1016/j.copbio.2007.04.006
Zhang Z, Houb Z, Yang C, Ma C, Tao F, Xu P (2011) Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8. Bioresour Technol 102:4111–4116. https://doi.org/10.1016/j.biortech.2010.12.064
Acknowledgements
The Director, CSIR-National Institute of Oceanography, Goa, India is thanked for providing all the lab facilities and funds to carry out this work. The Head of Biological Oceanography Division is thanked for his support and encouragement. Mr. R. Sreepada and Dr. Mamatha S.S. are thanked for their support in lab work. Ms. Sonam Pirankar and Mr. Aatish Fatefarkar are thanked for their assistance in work. VLS is thankful to the University Grant Commission (UGC, India) for providing her research fellowship. This has NIO contribution number 6500.
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VLS, SV, BDS conceived the idea. All authors conducted experiments. VLS, SV and BDS analysed the results. All authors reviewed the manuscript.
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Shinde, V.L., Suneel, V., Rathore, C. et al. Degradation of tarballs using associated bacterial consortia. 3 Biotech 10, 109 (2020). https://doi.org/10.1007/s13205-020-2095-8
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DOI: https://doi.org/10.1007/s13205-020-2095-8