Abstract
The action of FTO on myoblasts proliferation and differentiation and molecular mechanism underlying it were investigated by transfecting with FTO lentiviral overexpression vector and gene expression profile sequencing. Compared with the control group, myoblasts with FTO transfection was significantly enhanced proliferation; the expression of MYOG and MYOD mRNA was significantly increased. In cells transfected with FTO, 129 differentially expressed genes were determined compared with control group, with 104 up-regulated and 25 down-regulated genes. Twelve pathways (Phagosome, Focal adhesion, Adrenergic signaling in cardiomyocytes, Endocytosis, Cardiac muscle contraction, Toll-like receptor, Ribosome, Tight junction, Regulation of actin cytoskeleton, Cytokine–cytokine receptor interaction, Adrenergic signaling in cardiomyocytes and MAPK) were significantly enriched. Eight genes known to be directly or indirectly related to skeletal muscle development (LAMA5, SPP1, CAV3, RASGRF1, FAK, PDGFB, PDGFRα, and RAC2) were enriched in the focal adhesion and expressed differentially. Altogether, these data suggested that FTO stimulated differentiation of myoblasts through regulation of eight genes enriched in the focal adhesion.
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We thank International Science Editing (https://www.international science editing. com) for editing this manuscript.
Funding
The research was supported by the Natural Science Fund project in Jiangsu (BK20191217), China Meat-type Chicken Research System (CARS-42-Z06), Key Laboratory of Poultry Genetics and Breeding in Jiangsu (JQLAB-ZZ-201705), and the Special Funds for Transformation of Scientific and Technological Projects in Jiangsu Province (BA2019049).
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Conceptualization: HH and SL; validation: CL and LL; data curation: ZL; formal analysis: QW and ZH; writing the original draft: HH; supervision: SL.
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The study project was conducted in accordance with the Guidelines for Experimental Animals formulated by the Ministry of Science and Technology (Beijing, China). All experimental programs were approved by the Science Research Department (in charge of animal welfare) of the Institute of Animal Sciences, CAAS (Beijing, China).
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Huang, H., Liu, L., Li, C. et al. Fat mass- and obesity-associated (FTO) gene promoted myoblast differentiation through the focal adhesion pathway in chicken. 3 Biotech 10, 403 (2020). https://doi.org/10.1007/s13205-020-02386-z
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DOI: https://doi.org/10.1007/s13205-020-02386-z