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Expression of short hairpin RNA (shRNA) targeting AC2 gene of Mungbean yellow mosaic India virus (MYMIV) reduces the viral titre in soybean

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Abstract

Mungbean yellow mosaic India virus (MYMIV) belonging to the family Geminiviridae and the genus Begomovirus is a severe pathogen of tropical legumes including soybean. The absence of genetically mapped loci conferring resistance together with the genetic diversity of begomoviruses infecting soybean warrants the utilization of RNA interference (RNAi) technology to develop virus resistance. However, viral suppressors of RNAi (VSRs) reduce the effectiveness of RNA silencing. Here, we report the effectiveness of Agrobacterium-mediated transient expression of shRNA, targeting a conserved region of AC2 ORF (a VSR) of MYMIV, in conferring virus resistance in soybean. Transient expression of shRNA showed progressive reduction of the viral titre estimated by the MYMIV-derived AC2 gene copy numbers from the initial inoculum by approximately 80-fold 20 days post-application. In addition, the newly emerging leaves exhibited symptom recovery. Thus, this study proves that AC2 of MYMIV is a potent target gene for obtaining RNAi-mediated virus resistance in soybean. Agro-infiltration-based delivery of shRNA was an efficient means of gene silencing and could pave way for the development of transgenic virus-resistant soybean genotype.

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Acknowledgements

Authors gratefully acknowledge Indian Council of Agricultural Research (ICAR) (DSR 1.24/12) for funding this investigation.

Funding

This work was supported by the Indian Council of Agricultural Research (ICAR) (Grant number DSR 1.24/12).

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Authors and Affiliations

  1. ICAR-Indian Institute of Soybean Research (ICAR-IISR), Indore, Madhya Pradesh, 452001, India

    Shunmugiah V. Ramesh & Maranna Shivakumar

  2. ICAR-Indian Agricultural Research Institute (ICAR-IARI), New Delhi, 110012, India

    Shelly Praveen

  3. School of Life Sciences, Devi Ahilya Vishwavidyalaya (DAVV), Indore, Madhya Pradesh, 452001, India

    Bhagat S. Chouhan & Suresh Chand

  4. ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI), Kasaragod, Kerala, 671 124, India

    Shunmugiah V. Ramesh

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  1. Shunmugiah V. Ramesh
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  2. Maranna Shivakumar
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  3. Shelly Praveen
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  4. Bhagat S. Chouhan
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  5. Suresh Chand
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Contributions

SVR conceived and designed the study; SVR and BSC performed the experiments; MS and SC contributed through intellectual inputs and reagents; SP designed viral quantification experiments; SVR, MS, and SP wrote the manuscript; all authors have read and approved the manuscript.

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Correspondence to Shunmugiah V. Ramesh.

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This article does not contain any studies with human or animal subjects performed by any of the authors. Authors declare that they have no competing interests.

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Ramesh, S.V., Shivakumar, M., Praveen, S. et al. Expression of short hairpin RNA (shRNA) targeting AC2 gene of Mungbean yellow mosaic India virus (MYMIV) reduces the viral titre in soybean. 3 Biotech 9, 334 (2019). https://doi.org/10.1007/s13205-019-1865-7

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