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Chloroplast proteins involved in drought stress response in selected cultivars of common bean (Phaseolus vulgaris L.)

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Abstract

One of the major cell organelles, whose functions are affected during drought stress are chloroplasts. In this study, chloroplast proteome under drought was studied in two cultivars of common bean (Phaseolus vulgaris L), Tiber and more sensitive to drought, Starozagorski čern, which were subjected to drought for 6 and 13 days. A comparative proteomic analysis with 2D-DIGE was performed on the isolated chloroplast proteins from leaves. Together, 44 proteins with changed abundance between control and stressed plants were identified with LC–MS/MS from both cultivars. The majority of the identified proteins were involved in photosynthetic processes. The results showed a decrease in abundance in different structure components of photosystem I and II, and ATP synthase, which may indicate a suppression of light-dependent reactions by drought stress. Similar proteomic response for both cultivars after 6 and 13 days of drought was observed. Proteins with contrasting abundance patterns between the cultivars or proteins specific for only one cultivar, such as ferredoxin–NADP reductase, photosystem II stability/assembly factor HCF136, curvature thylakoid protein 1B, and plastidial membrane protein porin were pointed out as major identified proteins revealing differential abundance between the cultivars. Taken together, our results provide insight into the molecular response of chloroplasts in common bean under drought stress, whereas conclusions about the tolerance mechanisms require further studies.

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Acknowledgements

This work was supported by the Slovenian Research Agency (Z4-8223 and P4-0072).

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Authors and Affiliations

  1. Biotechnical Faculty, University of Ljubljana, 1000, Ljubljana, Slovenia

    Tanja Zadražnik

  2. Department of Molecular Biosciences, University of Oslo, 0316, Oslo, Norway

    Anders Moen

  3. Agricultural Institute of Slovenia, 1000, Ljubljana, Slovenia

    Jelka Šuštar-Vozlič

Authors
  1. Tanja Zadražnik
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  2. Anders Moen
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  3. Jelka Šuštar-Vozlič
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Contributions

TZ and JŠV designed the experiment. TZ performed the proteomic experiment and the data analysis. AM performed mass spectrometry analysis and protein identification. All authors participated in manuscript writing and approved the manuscript.

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Correspondence to Tanja Zadražnik.

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The authors declare no conflict of interest.

Electronic supplementary material

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13205_2019_1862_MOESM1_ESM.xlsx

Experimental design for 2D-DIGE. Four replicates from each control and drought-stressed protein samples from two cultivars subjected to drought for 6 and 13 days were labelled and combined for 2D-DIGE

Supplementary material 1 (XLSX 11 kb)

The detailed list of identified proteins with peptides and identification parameters

Supplementary material 2 (XLSX 105 kb)

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Zadražnik, T., Moen, A. & Šuštar-Vozlič, J. Chloroplast proteins involved in drought stress response in selected cultivars of common bean (Phaseolus vulgaris L.). 3 Biotech 9, 331 (2019). https://doi.org/10.1007/s13205-019-1862-x

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  • DOI: https://doi.org/10.1007/s13205-019-1862-x

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