Abstract
In the present study, seven axenic fresh water microchlorophytes were isolated and identified as Tetradesmus dimorphus (NEIST BT-1), Chlorella sorokiniana (NEIST BT-2), Desmodesmus sp. (NEIST BT-10), Selenastrum sp. (NEIST BT-A6), Tetradesmus obliquus (NEIST BT-A1), Tetradesmus sp. (NEIST BT-A10), and Asterarcys sp. (NEIST BT-A15) based on morphological and molecular characterization. Their potential to be used as biodiesel feedstock was evaluated depending on their growth characteristics and lipid profiles. Among the seven isolates, NEIST BT-2 was found to be the most promising candidate owing to its high biomass yield (2.09 ± 0.037 g L−1) and lipid productivity (107.60 ± 10.175 mg L−1 day−1). The gas chromatography analysis confirmed the presence of significant amounts of palmitic acid, linoleic acid, linolenic acid, and oleic acid in the isolate which are some of the major constituents of any biodiesel. The predictive models showed that the biodiesel from this isolate has ideal fuel properties which comply with the ASTM D6751 and EN 14214 specifications. These findings demonstrate that NEIST BT-2 can be used as a prospective candidate for consideration of large-scale biodiesel production.
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Abbreviations
- ASTM:
-
American society for testing and materials
- BP:
-
Biomass productivity
- CFPP:
-
Cold filter plugging point
- CN:
-
Cetane number
- CP:
-
Cloud point
- DCM:
-
Dry cell mass
- DMSO:
-
Dimethyl sulfoxide
- DU:
-
Degree of unsaturation
- EN:
-
European Standards
- FAME:
-
Fatty acid methyl ester
- FID:
-
Flame ionization detector
- GCV:
-
Gross calorific value
- IV:
-
Iodine value
- LC:
-
Lipid content
- LCSF:
-
Long-chain saturated factor
- LP:
-
Lipid productivity
- MUFA:
-
Monounsaturated fatty acid
- NCV:
-
Net calorific value
- OD:
-
Optical density
- PUFA:
-
Polyunsaturated fatty acid
- SEM:
-
Scanning electron microscope
- SFA:
-
Saturated fatty acid
- SV:
-
Saponification value
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Acknowledgements
This work was supported with funds from Council of Scientific & Industrial Research, New Delhi in the form of research grants from BioEn (CSC-0116, FTT-2001). Technical support was received from Dr. B. L. A Prabhavathi Devi, Senior Principal Scientist, CSIR-Indian Institute of Chemical Technology, Hyderabad for performing the fatty acid profiling of the strain using gas chromatography.
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Sehgal, A., Goswami, K., Pal, M. et al. Morpho-taxonomic, genetic, and biochemical characterization of freshwater microalgae as potential biodiesel feedstock. 3 Biotech 9, 137 (2019). https://doi.org/10.1007/s13205-019-1664-1
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DOI: https://doi.org/10.1007/s13205-019-1664-1