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Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis

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Abstract

The present study reports the effect of treatment of coconut embryogenic structure explants (derived from embryogenic callus) with the calcium ionophore A23187 (0, 1, 5, 10 µM) to promote somatic embryogenesis under in vitro conditions. The results showed no significant increase in the percentage of explants forming embryogenic callus, but with 1 µM ionophore there were significant increases in the formation of embryogenic structures per callus (2.8-fold), of somatic embryos per callus (1.5-fold) and also a greater absolute number (1.5-fold) of developing plantlets per callus. The ionophore treatment also promoted a change of pattern of the expression of the CnSERK gene during embryogenic callus formation. It is proposed that if the use of ionophore A23187 treatment is coupled with an embryogenic callus multiplication process there could be a potentially greater increase in the efficiency of the formation of somatic embryos and plantlets of coconut.

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References

  • Altamura MM, Rovere FD, Fattorini L, D’Angeli S, Falasca G (2016) Recent advances on genetic and physiological bases on in vitro somatic embryo formation. In: Germanà MA, Lambardi M (eds) In vitro embryogenesis in higher plants. Humana Press, New York, p 558

    Google Scholar 

  • Anil VS, Rao KS (2000) Calcium-mediated signaling during sandalwood somatic embryogenesis. Role for exogenous calcium as second messenger. Plant Physiol 123:1301–1312. https://doi.org/10.1104/pp.123.4.1301

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Azpeitia A, Chan JL, Sáenz L, Oropeza C (2003) Effect of 22(S)-homobrassinolide in somatic embryogenesis in plumule explants of Cocos nucifera (L.) cultured in vitro. J Hortic Sci Biotech 78:591–596. https://doi.org/10.1080/14620316.2003.11511669

    Article  CAS  Google Scholar 

  • Batugal P, Rao Ramanatha V, Oliver J (2005) Coconut genetic resources. International Plant Genetic Resources Institute - Regional office for Asia, the Pacific and Oceania. IPGRI-APO, Serdang

  • Buffard-Morel J, Verdeil JL, Pannetier C (1992) Embryogenèse somatique du cocotier (Cocos nucifera L.) à partir d´explants foliaires: étude histologique. Can J Bot 70:735–741

    Article  Google Scholar 

  • Chan JL, Saénz L, Talavera C, Hornung R, Robert M, Oropeza C (1998) Regeneration of coconut (Cocos nucifera L.) from plumule explants through somatic embryogenesis. Plant Cell Rep 17:515–521. https://doi.org/10.1007/s002990050434

    Article  CAS  Google Scholar 

  • Dedkova EN, Sigova AA, Zinchenko VP (2000) Mechanism of action of calcium ionophores on intact cells: ionophore-resistant cells. Membr Cell Biol 13:357–368

    CAS  PubMed  Google Scholar 

  • Du L, Poovaiah BW (2005) Ca2+/calmodulin is critical for brassinosteroid biosynthesis and plant growth. Nature 437:741–745. https://doi.org/10.1038/nature03973

    Article  CAS  PubMed  Google Scholar 

  • FAO (2016) FAOSTAT. http://www.fao.org/faostat/. Accessed 15 Jan 2018

  • Filippov M, Miroshnichenko D, Vernikovskaya D, Dolgov S (2006) The effect of auxins, time exposure to auxin and genotypes on somatic embryogenesis from mature embryos of wheat. Plant Cell Tiss Org 84:213–222. https://doi.org/10.1007/s11240-005-9026-6

    Article  CAS  Google Scholar 

  • Fisher DB (1968) Protein staining of ribboned epon sections for light microscopy. Histochemie 16:92–96

    Article  CAS  PubMed  Google Scholar 

  • Gaj MD (2004) Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana (L.) Heynh. Plant Growth Regul 43:27–47

    Article  CAS  Google Scholar 

  • George EF, Hall MA, De Klerk G (eds) (2008) Plant propagation by tissue culture. The background, 3rd edn, vol. 1. Springer, Dordrecht

    Google Scholar 

  • Gurr GM, Johnson AC, Ash GJ, Wilson BAL, Ero MM, Pilotti CA, Dewhurst CF, You MS (2016) Coconut lethal yellowing diseases: a phytoplasma threat to palms of global economic and social significance. Front Plant Sci 7:1–21. https://doi.org/10.3389/fpls.2016.01521

    Article  Google Scholar 

  • Hecht V, Vielle-calzada J-P, Hartog MV, Schmidt EDL, Boutilier K, Grossniklaus U, de Vries SC (2001) The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol 127:803–816. https://doi.org/10.1104/pp.010324.In

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hita O, Gallego P, Villalobos N, Lanas I, Blazquez A, Martin JP, Fernández J, Martin L, Guerra H (2003) Improvement of somatic embryogenesis in Medicago arborea. Plant Cell Tiss Org 72:13–18. https://doi.org/10.1023/A:1021297902139

    Article  CAS  Google Scholar 

  • Hornung R, Verdeil JL (1999) Somatic embryogenesis in coconut from immature inflorescence explants. In: Oropeza C, Verdeil JL, Ashburner GR et al (eds) Current advances in coconut biotechnology. Kluwer Academic Publishers, The Netherlands, pp 297–308

    Chapter  Google Scholar 

  • Jiao Y, Li Z, Xu K, Guo Y, Zhang C, Li T, Jiang Y, Liu G, Xu Y (2018) Study on improving plantlet development and embryo germination rates in in vitro embryo rescue of seedless grapevine. New Zeal J Crop Hort 46:39–53. https://doi.org/10.1080/01140671.2017.1338301

    Article  CAS  Google Scholar 

  • Kiselev KV, Gorpenchenko TY, Tchernoded GK, Dubrovina AS, Grischenko OV, Bulgakov VP, Zhuravlev YN (2008) Calcium-dependent mechanism of somatic embryogenesis in Panax ginseng cell cultures expressing the rolC oncogene. Mol Biol 42:243–252. https://doi.org/10.1134/S0026893308020106

    Article  CAS  Google Scholar 

  • Leljak-Levanic D, Bauer N, Mihaljevic S, Jelaska S (2004) Somatic embryogenesis in pumpkin (Cucurbita pepo L.): Control of somatic embryo development by nitrogen compounds. J Plant Physiol 161:229–236

    Article  CAS  PubMed  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2–∆∆CT method. Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262

    Article  CAS  Google Scholar 

  • Mahalakshmi A, Singla B, Kurana JP, Khurana P (2007) Role of calcium-calmodulin in auxin-induced somatic embryogenesis in leaf base cultures of wheat (Triticum aestivum var. HD 2329). Plant Cell Tiss Org 88:167–174. https://doi.org/10.1007/s11240-006-9186-z

    Article  CAS  Google Scholar 

  • Montero-Cortés M, Rodriguez-Paredes F, Burgeff C, Pérez-Núñez T, Córdova I, Oropeza C, Verdeil JL, Sáenz L (2010a) Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm. Plant Cell Tiss Org 102:251–258. https://doi.org/10.1007/s11240-010-9714-8

    Article  CAS  Google Scholar 

  • Montero-Cortés M, Sáenz L, Córdova I, Quiroz A, Verdeil JL, Oropeza C (2010b) GA3 stimulates the formation and germination of somatic embryos and the expression of a KNOTTED-like homeobox gene of Cocos nucifera (L.). Plant Cell Rep 29:1049–1059. https://doi.org/10.1007/s00299-010-0890-0

    Article  CAS  PubMed  Google Scholar 

  • Montero-Cortés M, Chan-Rodríguez JL, Cordova-Lara I, Oropeza-Salín C, Sáenz-Carbonell L (2011a) Addition of benzyladenine to coconut explants cultured in vitro improves the formation of somatic embryos and their germination. Agrociencia 45:663–673

    Google Scholar 

  • Montero-Cortés M, Cordova I, Verdeil JL, Hocher V, Pech y Aké A, Sandoval A, Oropeza C, Sáenz L (2011b) GA3 induces expression of E2F-like genes and CDKA during in vitro germination of zygotic embryos of Cocos nucifera (L.). Plant Cell Tiss Org 107:461–470. https://doi.org/10.1007/s11240-011-9996-5

    Article  CAS  Google Scholar 

  • Moon HK, Kim YW, Hong YP, Park SY (2013) Improvement of somatic embryogenesis and plantlet conversion in Oplopanax elatus, an endangered medicinal woody plant. SpringerPlus 2:1–8. https://doi.org/10.1186/2193-1801-2-428

    Article  CAS  Google Scholar 

  • Nguyen QT, Bandupriya HDD, López-Villalobos A, Sisunandar S, Foale M, Adkins SW (2015) Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review. Planta 242:1059–1076. https://doi.org/10.1007/s00425-015-2362-9

    Article  CAS  PubMed  Google Scholar 

  • Ntushelo K, Harrison NA (2013) Palm phytoplasmas in the Caribbean Basin. Palms 57:93–100

    Google Scholar 

  • Oropeza C, Rillo E, Hocher V, Verdeil J (2005) Coconut micropropagation. In: Batugal P, Pao V, Oliver J (eds) Coconut genetic resources. IPGRI-APO, Serdang, pp 334–346

    Google Scholar 

  • Oropeza C, Sáenz L, Chan JL, Sandoval G, Pérez-Núñez T, Narváez M, Rodríguez G, Borroto C (2016) Coconut micropropagation in Mexico using plumule and floral explants. Int J Coconut R D 32

  • Pérez-Núñez MT, Chan JL, Sáenz L, González T, Verdeil JL, Oropeza C (2006) Improved somatic embryogenesis from Cocos nucifera (L.) plumule explants. In Vitro Cell Dev-Pl 42:37–43. https://doi.org/10.1079/IVP2005722

    Article  Google Scholar 

  • Pérez-Núñez MT, Souza R, Sáenz L, Chan JL, Zúñiga-Aguilar JJ, Oropeza C (2009) Detection of a SERK-like gene in coconut and analysis of its expression during the formation of embryogenic callus and somatic embryos. Plant Cell Rep 28:11–19. https://doi.org/10.1007/s00299-008-0616-8

    Article  CAS  PubMed  Google Scholar 

  • Prades A, Salum UN, Pioch D (2016) New era for the coconut sector. What prospects for research? OCL 23:1–4. https://doi.org/10.1051/ocl/2016048

    Article  Google Scholar 

  • Prakash MG, Gurumurthi K (2010) Effects of type of explant and age, plant growth regulators and medium strength on somatic embryogenesis and plant regeneration in Eucalyptus camaldulensis. Plant Cell Tiss Org 100:13–20. https://doi.org/10.1007/s11240-009-9611-1

    Article  CAS  Google Scholar 

  • Quint M, Gray WM (2006) Auxin signaling. Curr Opin Plant Biol 9:448–453. https://doi.org/10.1016/j.pbi.2006.07.006

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rajesh MK, Fayas TP, Naganeeswaran S, Rachana KE, Bhavyashree U, Sajini KK, Karun A (2016) De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing. Protoplasma 253:913–928. https://doi.org/10.1007/s00709-015-0856-8

    Article  CAS  PubMed  Google Scholar 

  • Ramakrishna A, Giridhar P, Ravishankar GA (2011) Calcium and calcium ionophore A23187 induce high-frequency somatic embryogenesis in cultured tissues of Coffea canephora P ex Fr. In Vitro Cell Dev-Pl 47:667–673. https://doi.org/10.1007/s11627-011-9372-5

    Article  CAS  Google Scholar 

  • Roolant L (2014) Why coconut water is now a one billion industry. https://transferwise.com/blog/2014-05/why-coconut-water-is-now-a-1-billion-industry/. Accessed 2 Nov 2015

  • Sáenz L, Chan JL, Souza R, Hornung R, Rilo E, Verdeil JL, Oropeza C (1999) Somatic embryogenesis and regeneration in coconut from plumular explants. In: Oropeza C, Verdeil JL, Ashburner GR, Cardeña R, Santamaría JM (eds) Current advances in coconut biotechnology. Springer International Publishing, Netherlands, pp 309–319

    Chapter  Google Scholar 

  • Sáenz L, Azpeitia A, Chuc-Armendariz B, Chan JL, Verdeil JL, Hocher V, Oropeza C (2006) Morphological and histological changes during somatic embryo formation from coconut plumule explants. In Vitro Cell Dev-Pl 42:19–25. https://doi.org/10.1079/IVP2005728

    Article  Google Scholar 

  • Sáenz L, Herrera-Herrera G, Uicab-Ballote F, Chan JL, Oropeza C (2010) Influence of form of activated charcoal on embryogenic callus formation in coconut (Cocos nucifera). Plant Cell Tiss Org 100:301–308. https://doi.org/10.1007/s11240-009-9651-6

    Article  Google Scholar 

  • Sáenz L, Montero-Cortés M, Pérez-Núñez T, Azpeitia-Morales A, Andrade-Torres A, Córdova-Lara I, Chan-Rodríguez JL, Sandoval-Cancino G, Rivera-Solís G, Oropeza-Salín C (2016) Somatic embryogenesis in Cocos nucifera L. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Somatic embryogenesis: fundamental aspects and applications. Springer International Publishing, Switzerland, pp 297–318

    Chapter  Google Scholar 

  • Sandoval-Cancino G, Sáenz L, Chan JL, Oropeza C (2016) Improved formation of embryogenic callus from coconut immature inflorescence explants. In Vitro Cell Dev-Pl 52:367–378. https://doi.org/10.1007/s11627-016-9780-7

    Article  CAS  Google Scholar 

  • Schmidt EDL, Guzzo F, Toonen M, de Vries SC (1997) A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124:2049–2062. https://doi.org/10.1093/pcp/pci031

    Article  CAS  PubMed  Google Scholar 

  • Somleva MN, Schmidt EDL, de Vries SC (2000) Embryogenic cells in Dactylis glomerata L. (Poaceae) explants identified by cell tracking and by SERK expression. Plant Cell Rep 19:718–726. https://doi.org/10.1007/s002999900169

    Article  CAS  Google Scholar 

  • Takeda T, Inose H, Matsuoka H (2003) Stimulation of somatic embryogenesis in carrot by additional calcium. Biochem Eng J 14:143–148

    Article  CAS  Google Scholar 

  • Vanneste S, Friml J (2013) Calcium: the missing link in auxin action. Plants 2:650–675. https://doi.org/10.3390/plants2040650

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Verma SK, Das AK, Gantait S, Gurel S, Gurel E (2018) Influence of auxin and its polar transport inhibitor on the development of somatic embryos in Digitalis trojana. 3 Biotech 8:1–8. https://doi.org/10.1007/s13205-018-1119-0

    Article  Google Scholar 

  • Vidican TI, Cachita-Cosma D (2010) Studies regarding the influence of different wavelengths of LEDs light on regenerative and morphogenetic processes in in vitro cultures of Echinopsis chamaecereus f. lutea. Stud Univ Vasile Goldis Arad, Ser Stiint Vietii 20:41–45

    Google Scholar 

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Acknowledgements

The authors thanks, IA José Luis Chan Rodríguez, for the technical support and advice; Fomix-Yucatán (Mexico, YUC-2011-C09-169886) and Centro de Investgación Científica de Yucatán A.C, for partial funding; and to Consejo Nacional de Ciencia y Tecnología for a scholarship (242979) for Gustavo Alberto Rivera-Solís.

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  1. Centro de Investigación Científica de Yucatán (CICY), A.C., Unidad de Biotecnología, Calle 43 No. 130 x 32 y 34. Chuburná de Hidalgo, CP. 97205, Mérida, Yucatán, Mexico

    Gustavo Rivera-Solís, Luis Sáenz-Carbonell, María Narváez, Guillermo Rodríguez & Carlos Oropeza

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  1. Gustavo Rivera-Solís
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  2. Luis Sáenz-Carbonell
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  3. María Narváez
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  4. Guillermo Rodríguez
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Correspondence to Carlos Oropeza.

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Rivera-Solís, G., Sáenz-Carbonell, L., Narváez, M. et al. Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis. 3 Biotech 8, 366 (2018). https://doi.org/10.1007/s13205-018-1392-y

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