Abstract
Bacillus velezensis 157 was isolated from the bark of Eucommia ulmoides, and exhibited antagonistic activity against a broad spectrum of pathogenic bacteria and fungi. Moreover, B. velezensis 157 also showed various lignocellulolytic activities including cellulase, xylanase, α-amylase, and pectinase, which had the ability of using the agro-industrial waste (soybean meal, wheat bran, sugarcane bagasse, wheat straw, rice husk, maize flour and maize straw) under solid-state fermentation and obtained several industrially valuable enzymes. Soybean meal appeared to be the most efficient substrate for the single fermentation of B. velezensis 157. Highest yield of pectinase (19.15 ± 2.66 U g−1), cellulase (46.69 ± 1.19 U g−1) and amylase (2097.18 ± 15.28 U g−1) was achieved on untreated soybean meal. Highest yield of xylanase (22.35 ± 2.24 U g−1) was obtained on untreated wheat bran. Here, we report the complete genome sequence of the B. velezensis 157, composed of a circular 4,013,317 bp chromosome with 3789 coding genes and a G + C content of 46.41%, one circular 8439 bp plasmid and a G + C content of 40.32%. The genome contained a total of 8 candidate gene clusters (bacillaene, difficidin, macrolactin, butirosin, bacillibactin, bacilysin, fengycin and surfactin), and dedicates over 15.8% of the whole genome to synthesize secondary metabolite biosynthesis. In addition, the genes encoding enzymes involved in degradation of cellulose, xylan, lignin, starch, mannan, galactoside and arabinan were found in the B. velezensis 157 genome. Thus, the study of B. velezensis 157 broadened that B. velezensis can not only be used as biocontrol agents, but also has potentially a wide range of applications in lignocellulosic biomass conversion.
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Acknowledgements
This research was supported by The platform of comprehensive utilization of straw resources in Jilin Province (2014c-1) and the National High-Tech R&D Program of China (863 program) (2013AA102806), Science and Technology Development Program of Jilin Province (20160519011JH), Special Funds for Industrial Innovation of Jilin Province (2016C063), National Key R&D program of China (2017YFD0501000).
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Supplementary material 1 Figure S1. The decoloring circles of Bacillus velezensis 157 on selective mediums: a Congo red on CMC-amended agar plate, b Pectin agar plate, c the crude extract of Bacillus velezensis 157 (200 μL) was poured into the wells in Xylan agar plate, which was made with a gel cutter d Starch agar was overlayed with KI solution. Figure S2. Gel electrophoresis of native plasmid of Bacillus velezensis 157. Figure S3. Venn diagram of the four Bacillus velezensis strains based on protein cluster analysis, comparing the shared and unique protein clusters with each other (DOC 1192 kb)
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Chen, L., Gu, W., Xu, Hy. et al. Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF. 3 Biotech 8, 114 (2018). https://doi.org/10.1007/s13205-018-1125-2
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DOI: https://doi.org/10.1007/s13205-018-1125-2