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Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers

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Abstract

Costus pictus, belonging to the family Costaceae, is one of the valuable medicinal plants with its anti-diabetic property. Despite ever-increasing demand from the pharmaceutical industry, this species is being less exploited at molecular level. Hence, an effort has been made in the present study to characterize the 15 accessions of C. pictus collected from different geographical regions of India through random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. A total of 25 RAPD and 20 ISSR primers were used in the present study. The RAPD analysis generated 343 loci, of which 124 were polymorphic with an average of 4.96 loci per primer. While, ISSR primers produced 177 loci, of which 77 were polymorphic with an average of 3.85 loci per primer. The similarity coefficients ranged from 0.86–0.99, 0.84–0.95 and 0.86–0.96 for RAPD, ISSR and combined RAPD-ISSR, respectively. The UPGMA dendrogram generated using these data showed low level of divergence among the accessions from South and West regions. Further, accession-specific bands were also revealed by RAPD and ISSR markers which might be contributed to specific trait. This investigation was an understanding of genetic variation within the C. pictus accessions. The present finding indicates that both the marker tools RAPD and ISSR combined or individually can be used in determining the genetic relationship between the accessions. It may be concluded that data of hereditary differences appeared among the C. pictus accessions could be utilized for their conservation and reproducing programs.

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Acknowledgements

The authors are sincerely thankful to the Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari, for providing all the necessary facilities to conduct this research.

Author contributions

AN Collected samples and conducted main experiment; PP Analyzed the data and critically reviewed the manuscript; RK Reviewed the manuscript and provided all the facilities to conduct this experiment; JMP Helped in experimental setup and analyzed the data.

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Authors and Affiliations

  1. C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Tarsadia, Surat, Gujarat, 396350, India

    Ami Naik & R. Krishnamurthy

  2. ICAR-Directorate of Medicinal and Aromatic Plants Research, Boriavi, Gujarat, 387310, India

    Pravin Prajapat

  3. Zandu Foundation for Health Care, Pardi, Valsad, Gujarat, 396145, India

    J. M. Pathak

Authors
  1. Ami Naik
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  2. Pravin Prajapat
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  3. R. Krishnamurthy
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  4. J. M. Pathak
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Correspondence to R. Krishnamurthy.

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Naik, A., Prajapat, P., Krishnamurthy, R. et al. Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers. 3 Biotech 7, 70 (2017). https://doi.org/10.1007/s13205-017-0667-z

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