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Silencing NAF-1 represses the proliferation and motility of PDAC cells through inhibiting HIF-1α

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Abstract

Objective

To investigate the effect of NAF-1 on the proliferation and motility of PDAC cells and the molecular mechanism.

Methods

siRNA was used to knock down the expression of NAF1 in pancreatic cancer cells; qRT-PCR and western blot were performed to detect the level of NAF-1, HIF-1α, or EMT markers. The effects of NAF-1on cell proliferation and motility were assessed via colony formation, flow cytometry, wound healing assay, and transwell assays.

Results

NAF-1 was mainly localized in the cytoplasm according to the results of immunofluorescence. NAF-1 knockdown significantly repressed the proliferation and induced apoptosis of pancreatic cell. Besides, NAF-1 deficiency in Panc-1 cells obviously impaired the migration, invasion and EMT process. Mechanistically, HIF-1α mediates the biological function of NAF-1.

Conclusions

Silencing NAF-1 significantly represses the proliferation, inducing the apoptosis of pancreatic cell, besides, NAF-1 play a key role in repressing the motility of PDAC cells through inhibiting HIF-1α.

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References

  • Aroldi F, Bertocchi P, Savelli G, Rosso E, Zaniboni A (2016) Pancreatic cancer: new hopes after first line treatment. World J Gastrointest Oncol 8(9):682–687

    Article  Google Scholar 

  • Chen YF, Wu CY, Kirby R, Kao CH, Tsai TF (2010) A role for the CISD2 gene in lifespan control and human disease. Ann N Y Acad Sci 1201:58–64

    Article  CAS  Google Scholar 

  • Chen K, Cheng L, Qian W et al (2018) Itraconazole inhibits invasion and migration of pancreatic cancer cells by suppressing TGF-beta/SMAD2/3 signaling. Oncol Rep 39(4):1573–1582

    Article  CAS  Google Scholar 

  • Cheng L, Yan B, Chen K et al (2018) Resveratrol-induced downregulation of NAF-1 enhances the sensitivity of pancreatic cancer cells to gemcitabine via the ROS/Nrf2 signaling pathways. Oxid Med Cell Longev 2018:9482018

    Article  Google Scholar 

  • Darash-Yahana M, Pozniak Y, Lu M et al (2016) Breast cancer tumorigenicity is dependent on high expression levels of NAF-1 and the lability of its Fe-S clusters. Proc Natl Acad Sci USA 113(39):10890–10895

    Article  CAS  Google Scholar 

  • Holt SH, Darash-Yahana M, Sohn YS et al (2016) Activation of apoptosis in NAF-1-deficient human epithelial breast cancer cells. J Cell Sci 129(1):155–165

    CAS  Google Scholar 

  • Leake I (2014) Pancreatic cancer: surprising role for fibrosis. Nat Rev Gastroenterol Hepatol 11(7):396

    Article  Google Scholar 

  • Li SM, Chen CH, Chen YW et al (2017) Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma. Sci Rep 7(1):11893

    Article  Google Scholar 

  • Liu L, Xia M, Wang J, Zhang W, Zhang Y, He M (2014) CISD2 expression is a novel marker correlating with pelvic lymph node metastasis and prognosis in patients with early-stage cervical cancer. Med Oncol 31(9):183

    Article  Google Scholar 

  • Masamune A, Kikuta K, Watanabe T, Satoh K, Hirota M, Shimosegawa T (2008) Hypoxia stimulates pancreatic stellate cells to induce fibrosis and angiogenesis in pancreatic cancer. Am J Physiol Gastrointest Liver Physiol 295(4):G709–G717

    Article  CAS  Google Scholar 

  • Qian J, Bai H, Gao Z et al (2016) Downregulation of HIF-1alpha inhibits the proliferation and invasion of non-small cell lung cancer NCI-H157 cells. Oncol Lett 11(3):1738–1744

    Article  CAS  Google Scholar 

  • Qin T, Cheng L, Xiao Y et al (2020) NAF-1 inhibition by resveratrol suppresses cancer stem cell-like properties and the invasion of pancreatic cancer. Front Oncol 10:1038

    Article  Google Scholar 

  • Siegel RL, Miller KD, Fuchs HE, Jemal A (2021) Cancer statistics, 2021. CA Cancer J Clin 71(1):7–33

    Article  Google Scholar 

  • Wang L, Ouyang F, Liu X et al (2016) Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway. Oncotarget 7(4):3791–3805

    Article  Google Scholar 

  • Wu S, Cheng Z, Yu L, Song W, Tao Y (2011) Expression of CD82/KAI1 and HIF-1alpha in non-small cell lung cancer and their relationship to vasculogenic mimicry. Zhongguo Fei Ai Za Zhi 14(12):918–925

    Google Scholar 

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Authors and Affiliations

  1. Medical Ultrasound Laboratory, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710004, China

    Xin Lu, Lirong Wang, Miao Li, Qi Zhou & Jue Jiang

Authors
  1. Xin Lu
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  2. Lirong Wang
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  3. Miao Li
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  4. Qi Zhou
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  5. Jue Jiang
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Corresponding authors

Correspondence to Qi Zhou or Jue Jiang.

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The authors declare that they have no conflict of interest.

Ethical approval

This research does not contain any studies with human participants or animals performed by any of the authors.The cell line used in this paper which was purchased from a commercial institute in China. Our research on PDAC cells has been approved by the Ethics committee of Second Affiliated Hospital of Xi’an Jiaotong University.

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Lu, X., Wang, L., Li, M. et al. Silencing NAF-1 represses the proliferation and motility of PDAC cells through inhibiting HIF-1α. Appl Nanosci 13, 2977–2984 (2023). https://doi.org/10.1007/s13204-022-02360-6

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  • DOI: https://doi.org/10.1007/s13204-022-02360-6

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