Abstract
The present research work depicts a cinch and economical synthesis of silver nanoparticles (AgNPs) phytofabricated utilizing leaf extract of Simarouba glauca and aerial extract of Celastrus paniculatus. Fourier transform infrared spectroscopy (FTIR) analysis displayed the synthesized AgNPs capped with bio-constituents existent in these medicinal plants marked by their functional groups. The morphological features of prepared NPs were characterized by employing X-ray Diffraction (XRD), Scanning Electron Microscope with Energy Dispersive spectra (SEM–EDX) and Transmission Electron Microscope (TEM) analysis illustrated their spherical shape and ~ 40 nm particle size. Green synthesized AgNPs were assessed for their antioxidant potential by free radical scavenging assays; and antibacterial efficacy. The phytofabricated AgNPs were found to induce cell growth arrest in MCF-7 and HT-29 cell lines with an IC50 value of 70.84 ± 0.67 and 158.24 ± 0.89 µg/mL for AgNPs prepared using S. glauca (Ag-SG) while 207.19 ± 0.64 and 221.22 ± 0.57 µg/mL for AgNPs synthesized using C. paniculatus (Ag-CP) in dose-proportional relation. The same samples could not effectively prohibit the growth of immortalized normal human breast epithelial cell lines (MCF-10A). The cytotoxicity induced by apoptosis was further confirmed by fluorescent images. The synthesized nanoparticles also demonstrated less hemolysis efficiency and are evidenced by SEM images. The analyses indicate the potent antioxidant and antiproliferative capacity of stable green synthesized AgNPs derived from the mentioned exorbitant curative herbs.
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Acknowledgements
The authors would like to acknowledge the assistance provided by the Plant Taxonomist, Dr. K. Ravikumar, Head of the Department, Centre of Conservation of Medicinal Resources, University of Trans-Disciplinary Health Sciences and Technology, Bangalore, in collecting the valuable plant materials and issuing the herbarium certificate. The authors are grateful to Dr. Takashi Morii, Dr. Arivazhagan Rajendran, and Dr. Shun Nakano, Institute of Advanced Energy, Kyoto University, Uji, Japan, for helping to carry out TEM analysis. Authors thank Vision Group on Science and Tecnology, Department of Information Technology, Biotechnology and Science & Technology, Government of Karnataka for financial assistance to Department of Applied Science (Nanotechnolgy), Visvesvaraya Technological University under KFIST Level-I scheme (GRD number: 479).
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Giridasappa, A., Ismail, S.M., Rangappa, D. et al. Antioxidant, antiproliferative and antihemolytic properties of phytofabricated silver nanoparticles using Simarouba glauca and Celastrus paniculatus extracts. Appl Nanosci 11, 2561–2576 (2021). https://doi.org/10.1007/s13204-021-02084-z
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DOI: https://doi.org/10.1007/s13204-021-02084-z