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Anti-oxidant, anti-fungal and cytotoxic effects of silver nanoparticles synthesized using marine fungus Cladosporium halotolerans

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Abstract

This study is to implement an easy and greener method for the preparation of silver nanoparticles (AgNPs) using fungal extract and investigate their antioxidant (DPPH), antifungal (Aspergillus niger) and cytotoxic effect against human breast cancer cell line MCF-7. The synthesized AgNPs were characterized by several analytical techniques, such as UV–Vis spectrophotometer (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). The results of analyses using spectroscopy (UV and FTIR) and diffraction (XRD) were compared and discussed with available literature. Electron microscopic techniques, such as SEM and TEM, illustrated spherical AgNPs. The inhibition of free radical DPPH by AgNPs was significant. The cytotoxicity of AgNPs was dose-dependent, and therefore, an increase in cell death was obtained with increasing concentrations of AgNPs. The IC50 values were found to be 34.27 µL/mL for the AgNPs and 17.69 µL/mL for the standard drug, Cisplatin. According to the knowledge of the authors and published literature, this is the first-ever report on the synthesis of AgNPs using the fungus Cladosporium halotolerans and evaluation of their biological activities.

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References

  • Adeeyo AO, Odiyo JO (2018) Biogenic synthesis of silver nanoparticle from mushroom exopolysaccharides and its potentials in water purification. Open Chem 5:64–75

    Article  Google Scholar 

  • Ahluwalia V, Kumar J, Sisodia R, Shakil NA, Walia S (2014) Green synthesis of silver nanoparticles by Trichoderma harzianum and their bioefficacy evaluation against Staphylococcus aureus and Klebsiella pneumonia. Ind Crops Prod 2014(55):202–206

    Article  Google Scholar 

  • Akintelu SA, Bo Y, Folorunso AS (2020) A review on synthesis, optimization, mechanism, characterization, and antibacterial application of silver nanoparticles synthesized from plants. J Chem. https://doi.org/10.1155/2020/3189043

  • Al-Ansari MM, Al-Dahmash ND, Ranjitsingh AJA (2021) Synthesis of silver nanoparticles using gum Arabic: evaluation of its inhibitory action on Streptococcus mutans causing dental caries and endocarditis. J Infect Public Health 14:324–330

    Article  Google Scholar 

  • Al-Otibi F, Perveen K, Al-Saif NA, Alharbi RI, Bokhari NA, Albasher G, Al-Otaibi RM, Al-Mosa MA (2021) Biosynthesis of silver nanoparticles using Malva parviflora and their antifungal activity. Saudi J Biol Sci 28:2229–2235

    Article  CAS  Google Scholar 

  • Ameen F, Alshehrei F (2017) Biodegradation optimization and metabolite elucidation of reactive red 120 by four different Aspergillus species isolated from soil contaminated with industrial effluent. Ann Microbiol 67:303–312

    Article  CAS  Google Scholar 

  • Antony JJ, Sivalingam P, Siva D, Kamalakkannan S, Anbarasu K, Sukirtha R, Krishnan M, Achiraman S (2011) Comparative evaluation of antibacterial activity of silver nanoparticles synthesized using Rhizophora apiculata and glucose. Coll Surf B 88:134–140

    Article  CAS  Google Scholar 

  • Assadi Z, Emtiazi G, Zarrabi A (2018) Hyperbranched polyglycerol coated on copper oxide nanoparticles as a novel core-shell nano-carrier hydrophilic drug delivery model. J Mol Liq 250:375–380

    Article  CAS  Google Scholar 

  • Azmath P, Baker S, Rakshith D, Satish S (2016) Mycosynthesis of silver nanoparticles bearing antibacterial activity. Saudi Pharm J 24:140–146

    Article  Google Scholar 

  • Babu PR, Sarma VV (2019) Fungi as promising biofuel resource. In: Singh JS, Singh DP (eds) New and Future Developments in Microbial Biotechnology and Bioengineering. Elsevier. https://doi.org/10.1016/c2017-0-03932-0

  • Balaji DS, Basavaraja S, Deshpande R, Mahesh DB, Prabhakar BK, Venkataraman A (2009) Extracellular biosynthesis of functionalized silver nanoparticles by strains of Cladosporium cladosporioides fungus. Coll Surf B Biointerfaces 68:88–92

    Article  CAS  Google Scholar 

  • Bérdy J (2005) Bioactive microbial metabolites. J Antibiot 58:1–26. https://doi.org/10.1038/ja.2005.1

    Article  Google Scholar 

  • Bhangale HG, Bachhav SG, Nerkar DM, Sarode KM, Patil DR (2019) Study on optical properties of green synthesized silver nanoparticles for surface plasmon resonance. J Nanosci Tech 5:658–661

    Article  Google Scholar 

  • Chhangte V, Samuel L, Ayushi B, Manickam S, Bishwajit C, Samuel LRR (2021) Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature. RSC Adv 11:2804–2837

    Article  Google Scholar 

  • Du L, Xu Q, Huang M, Xian L, Feng JX (2015) Synthesis of small silver nanoparticles under light radiation by fungus Penicillium oxalicum and its application for the catalytic reduction of methylene blue. Mater Chem Phys 160:40–47

    Article  CAS  Google Scholar 

  • Elamawi RM, Al-Harbi RE, Hendi AA (2018) Biosynthesis and characterization of silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic fungi. Egypt J Biol Pest Control 28:28

    Article  Google Scholar 

  • Gade AK, Bonde P, Ingle AP, Marcato PD, Durán N, Rai MK (2008) Exploitation of Aspergillus niger for synthesis of silver nanoparticles. J Biobased Mater Bioenergy 2:243–247

    Article  Google Scholar 

  • Gnanasekar S, Balakrishnan D, Seetharaman P, Arivalagan P, Chandrasekaran R, Sivaperumal S (2020) Chrysin-anchored silver and gold nanoparticle-reduced graphene oxide composites for breast cancer therapy. ACS Appl Nano Mater 3:4574–4585

    Article  CAS  Google Scholar 

  • Govindappa M, Tejashree S, Thanuja V, Hemashekhar B, Srinivas C, Nasif O, Pugazhendhi A, Raghavendra VB (2021) Pomegranate fruit fleshy pericarp mediated silver nanoparticles possessing antimicrobial, antibiofilm formation, antioxidant, biocompatibility and anticancer activity. J Drug Deliv Sci Technol 61:102289

    Article  CAS  Google Scholar 

  • Goyal S, Ramawat KG, Mérillon J-M (2016) Different shades of fungal metabolites: an overview. Fungal Metab 1:29

    Google Scholar 

  • Huang H, Shan K, Liu J, Tao X, Periyasamy S, Durairaj S, Jiang Z, Jacob JA (2020) Synthesis, optimization and characterization of silver nanoparticles using the catkin extract of Piper longum for bactericidal effect against food-borne pathogens via conventional and mathematical approaches. Bioorg Chem 103:104230

    Article  CAS  Google Scholar 

  • Ijaz M, Zafar M, Tahir I (2020) Green synthesis of silver nanoparticles by using various extracts: a review. Inorg Nano Metal Chem. https://doi.org/10.1080/24701556.2020.1808680

    Article  Google Scholar 

  • Javed R, Zia M, Naz S, Aisida SO, Ain Nu, Ao Q (2020) Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects. J Nanobiotechnol 18:172

    Article  Google Scholar 

  • Kedare SB, Singh RP (2011) Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 48:412–422

    Article  CAS  Google Scholar 

  • Khan NT, Khan MJ, Jameel J, Jameel N, Rheman SUA (2017) An overview: biological organisms that serves as nanofactories for metallic nanoparticles synthesis and fungi being the most appropriate. Bioceram Dev Appl 7:101

    Article  Google Scholar 

  • Khandel P, Shahi SK (2018) Mycogenic nanoparticles and their bioprospective applications: current status and future challenges. J Nanostr Chem 8:369–391

    Article  CAS  Google Scholar 

  • Kumar V, Singh DK, Mohan S, Hasan SH (2016) Photo-induced biosynthesis of silver nanoparticles using aqueous extract of Erigeron bonariensis and its catalytic activity against Acridine Orange. J Photochem Photobiol, B 155:39–50

    Article  CAS  Google Scholar 

  • Lee SH, Jun BH (2019) Silver nanoparticles: synthesis and application for nanomedicine. Int j Mol Sci 20:E865

    Article  Google Scholar 

  • Letunic I, Bork P (2011) Interactive tree of life v2: online annotation and display of phylogenetic trees made easy. Nucleic Acids Res 39:W475–W478

    Article  CAS  Google Scholar 

  • LewisOscar F, Vismaya S, Arunkumar M, Thajuddin N, Dhanasekaran D, Nithya C (2016) Algal nanoparticles: synthesis and biotechnological potentials. In: Thajuddin N (ed) Algae-organisms for imminent biotechnology. IntechOpen. https://doi.org/10.5772/62909

  • Liu X, Shan K, Shao X, Shi X, He Y, Liu Z, Jacob JA, Deng L (2021) Nanotoxic effects of silver nanoparticles on normal HEK-293 cells in comparison to cancerous HeLa cell line. Int J Nanomed 16:753

    Article  Google Scholar 

  • Manan MA, Webb C (2020) Newly designed multi-stacked circular tray solid-state bioreactor: analysis of a distributed parameter gas balance during solid-state fermentation with influence of variable initial moisture content arrangements. Biores Bioprocess 7:16

    Article  Google Scholar 

  • Mariana GC, de Renata L (2019) Synthesis of silver nanoparticles mediated by fungi: a review. Front Bioeng Biotechnol 7:287

    Article  Google Scholar 

  • Marimuthu S, Antonisamy AJ, Malayandi S, Rajendran K, Tsai P-C, Pugazhendhi A, Ponnusamy VK (2020) Silver nanoparticles in dye effluent treatment: a review on synthesis, treatment methods, mechanisms, photocatalytic degradation, toxic effects and mitigation of toxicity. J Photochem Photobiolo B Biol 205:111823

    Article  CAS  Google Scholar 

  • Moteriya P, Chanda S (2018) Biosynthesis of silver nanoparticles formation from Caesalpinia pulcherrima stem metabolites and their broad spectrum biological activities. J Genetic Eng Biotechnol 16:105–113

    Article  Google Scholar 

  • Munajad A, Subroto C (2018) Fourier transform infrared (FTIR) spectroscopy analysis of transformer paper in mineral oil-paper composite insulation under accelerated thermal aging. Energies 11:364

    Article  Google Scholar 

  • Netala VR, Bethu MS, Pushpalatah B, Baki VB, Aishwarya S, Rao JV (2016) Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsismicrospora and evaluation of their antioxidant and anticancer activities. Int J Nanomed 11:5683–5696

    Article  CAS  Google Scholar 

  • Othman AM, Elsayed MA, Al-Balakocy NG, Hassan MM, Elshafei AM (2019) Biosynthesis and characterization of silver nanoparticles induced by fungal proteins and its application in different biological activities. J Genet Eng Biotechnol 17:8–8

    Article  Google Scholar 

  • Riaz M, Ismail M, Ahmad B, Zahid N, Jabbour G, Khan MS, Mutreja V, Sareen S, Rafiq A, Faheem M (2020) Characterizations and analysis of the antioxidant, antimicrobial, and dye reduction ability of green synthesized silver nanoparticles. Green Process Synth 9:693–705

    Article  Google Scholar 

  • Roy A, Bulut O, Some S, Mandal AK, Yilmaz MD (2019) Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Adv 9:2673–2702

    Article  CAS  Google Scholar 

  • Sathishkumar P, Johnson P, Raji V, Abdull RMY, Fuad A, Sadhasivam S, Ramasamy B, Thayumanavan P (2016) Anti-acne, anti-dandruff and anti-breast cancer efficacy of green synthesized silver nanoparticles using Coriandrum Sativum leaf extract. J Photochem Photobiol 163:69–76

    Article  CAS  Google Scholar 

  • Seerangaraj V, Sathiyavimal S, Shankar SN, Nandagopal JGT, Balashanmugam P, Al-Misned FA, Shanmugavel M, Senthilkumar P, Pugazhendhi A (2021) Cytotoxic effects of silver nanoparticles on Ruellia tuberosa: photocatalytic degradation properties against crystal violet and coomassie brilliant blue. J Environ Chem Eng 9:105088

    Article  CAS  Google Scholar 

  • Shanmuganathan R, Karuppusamy I, Saravanan M, Muthukumar H, Ponnuchamy K, Ramkumar VS, Pugazhendhi A (2019) Synthesis of silver nanoparticles and their biomedical applications-a comprehensive review. Curr Pharm Des 25:2650–2660

    Article  CAS  Google Scholar 

  • Sreelekha E, Bini G, Aswathi S, Sajina N, Beena M (2021) A comparative study on the synthesis, characterization, and antioxidant activity of green and chemically synthesized silver nanoparticles. BioNanoScience. https://doi.org/10.1007/s12668-021-00824-7

    Article  Google Scholar 

  • Su D (2017) Advanced electron microscopy characterization of nanomaterials for catalysis. Green Energy Environ 2:70–83

    Article  Google Scholar 

  • Suriyakalaa U, Antony JJ, Suganya S, Siva D, Sukirtha R, Kamalakkannan S, Pichiah PT, Achiraman S (2013) Hepatocurative activity of biosynthesized silver nanoparticles fabricated using Andrographis paniculata. Coll Surf B 102:189–194

    Article  CAS  Google Scholar 

  • Theivasanthi T, Alagar M (2011) Electrolytic synthesis and characterizations of silver nanopowder. arXiv preprint arXiv:1111.0260

  • Vahabi K, Mansoori GA, Karimi S (2011) Biosynthesis of silver nanoparticles by fungus Trichoderma reesei. Insci j 1:65–79

    Article  CAS  Google Scholar 

  • Verduzco-Oliva R, Gutierrez-Uribe JA (2020) Beyond enzyme production: solid state fermentation (SSF) as an alternative approach to produce antioxidant polysaccharides. Sustainability 12:495

    Article  CAS  Google Scholar 

  • Wen X, Wang Q, Dai T, Shao J, Wu X, Jiang Z, Jacob JA, Jiang C (2020) Identification of possible reductants in the aqueous leaf extract of mangrove plant Rhizophora apiculata for the fabrication and cytotoxicity of silver nanoparticles against human osteosarcoma MG-63 cells. Mat Sci Eng C 116:111252

    Article  CAS  Google Scholar 

  • Zhang XF, Liu ZG, Shen W, Gurunathan S (2016) Silver nanoparticles: synthesis, characterization, properties, applications and therapeutic approaches. Int J Mol Sci 17:1534

    Article  Google Scholar 

  • Zhang H, Jacob JA, Jiang Z, Xu S, Sun K, Zhong Z, Varadharaju N, Shanmugam A (2019) Hepatoprotective effect of silver nanoparticles synthesized using aqueous leaf extract of Rhizophora apiculata. Int J Nanomed 14:3517

    Article  CAS  Google Scholar 

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Acknowledgements

This project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number (13-BIO2290-02).

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Authors and Affiliations

  1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Fuad Ameen, Ali A. Al-Homaidan, Ahmed Al-Sabri & Abobakr Almansob

  2. Deanship of Scientific Research, King Saud University, Riyadh, 11451, Saudi Arabia

    Saleh AlNAdhari

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  1. Fuad Ameen
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  2. Ali A. Al-Homaidan
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  3. Ahmed Al-Sabri
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  4. Abobakr Almansob
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  5. Saleh AlNAdhari
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Correspondence to Fuad Ameen.

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Ameen, F., Al-Homaidan, A.A., Al-Sabri, A. et al. Anti-oxidant, anti-fungal and cytotoxic effects of silver nanoparticles synthesized using marine fungus Cladosporium halotolerans. Appl Nanosci 13, 623–631 (2023). https://doi.org/10.1007/s13204-021-01874-9

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  • DOI: https://doi.org/10.1007/s13204-021-01874-9

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