Abstract
The multi-component nanosystems based on the branched copolymer dextran-graft-polyacrylamide in an anionic form (D-g-PAAan) with gold nanoparticles (AuNPs), photosensitizer Chlorine e6 (Ce6), and chemotherapeutic agent Doxorubicin (Dox) were developed for use in complex breast cancer therapy. The synthesized nanosystems were characterized by Quasi Elastic Light Scattering and Transmission Electron Microscopy and tested against the malignant cells MCF-7/Dox resistant to Dox. It is found that the addition of Dox as the fourth component to the three-component nanosystem D-g-PAAan/AuNPs/Ce6 leads to aggregation and, as a result, to decrease in efficiency of tumor treatment. The way to reduce unwanted aggregation in the four-component nanosystem D-g-PAAan/AuNPs/Ce6/Dox is proposed. The new nanosystems are shown to be effective in overcoming Dox resistance and killing cancer cells.
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Data availability
The data that support the findings of this study are available from the corresponding author, Yuliia Kuziv, upon reasonable request.
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Acknowledgments
This publication is supported in part by the Ministry of the Education and Science of Ukraine: joint Ukrainian-Belarusian research and development projects, Project M/70-2020 “Design and physico-chemical properties of novel multicomponent nanosystems for the treatment and diagnostics of solid tumors” and by National Research Foundation of Ukraine, Project 2020.02/0022 “Plasmon hybrid nanosystems "metal-polymer-fluorophore" with enhanced optical response for photonics and biomedical applications”.
Funding
This publication is supported in part by the Ministry of the Education and Science of Ukraine: joint Ukrainian-Belarusian research and development projects, Project M/70–2020 “Design and physico-chemical properties of novel multicomponent nanosystems for the treatment and diagnostics of solid tumors” and by National Research Foundation of Ukraine, Project 2020.02/0022 “Plasmon hybrid nanosystems "metal-polymer-fluorophore" with enhanced optical response for photonics and biomedical applications”.
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Kutsevol, N., Kuziv, Y., Bezugla, T. et al. Application of new multicomponent nanosystems for overcoming doxorubicin resistance in breast cancer therapy. Appl Nanosci 12, 427–437 (2022). https://doi.org/10.1007/s13204-020-01653-y
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DOI: https://doi.org/10.1007/s13204-020-01653-y