Abstract
Aflatoxin M1 (AFM1) is a metabolite of carcinogenic aflatoxin B1 (AFB1) and appears in milk of dairy animals on ingestion of feed contaminated with AFB1. It has been reported to have affinity towards milk proteins, the exact mechanism of which is still unknown. In the present study, ATR-FTIR coupled with chemometrics is utilized to understand AFM1 interaction with milk proteins. The second order derivative spectra of the spectral window 1700–1600 cm−1 confirms the affinity of AFM1 towards milk proteins. The results of principal component analysis suggested that spectral window of 1700–1600 cm−1 is informative and provides an indication of the conformational changes brought by AFM1 in the secondary structure of milk proteins.
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Abbreviations
- AFM1 :
-
Aflatoxin M1
- AFB1 :
-
Aflatoxin B1
- PCA:
-
Principal component analysis
- FTIR-ATR:
-
Fourier transform infrared-attenuated total reflectance
- SIMCA:
-
Soft independent modeling of class analogies
- IR:
-
Infrared
- SMP:
-
Skimmed milk powder
- ATR:
-
Attenuated total reflectance
- DLATGS:
-
Deuterated lanthanum α alanine doped triglycine sulphate
- SG:
-
Savitzky Golay
- NIST:
-
National Institute of Standards and Technology
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Acknowledgements
The authors are grateful to Director of ICAR-National Dairy Research Institute, India for providing the facilities at National Referral Center of Milk Quality & Safety to carry out this research work.
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HCG Investigation, methodology, writing-original draft, visualization. NS Conceptualization, validation, helped in writing-original draft. RS formal analysis, resources, writing-review and editing. RS Supervision, project administration, writing-review and editing. KG Writing-review and editing, visualization, resources. BM Writing-review and editing, visualization, guidance.
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Harshitha, C.G., Sharma, N., Singh, R. et al. Interaction study of aflatoxin M1 with milk proteins using ATR-FTIR. J Food Sci Technol 60, 64–72 (2023). https://doi.org/10.1007/s13197-022-05587-x
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DOI: https://doi.org/10.1007/s13197-022-05587-x