Abstract
Clitoria ternatea or commonly known as ‘Butterfly pea’ has been used traditionally in Ayurvedic medicine in which various parts of the plants are used to treat health issues such as indigestion, constipation, arthritis, skin diseases, liver and intestinal problems. The flowers of C. ternatea are used worldwide as ornamental flowers and traditionally used as a food colorant. This paper reviews the recent advances in the extraction and biological activities of phytochemicals from C. ternatea flowers. The application of maceration or ultrasound assisted extraction greatly increased the yield (16–247% of increase) of phytochemicals from C. ternatea flowers. Various phytochemicals such as kaempferol, quercetin and myricetin glycosides as well as anthocyanins have been isolated from C. ternatea flowers. Clitoria ternatea flower extracts were found to possess antimicrobial, antioxidant, anti-inflammatory, cytotoxic and antidiabetic activities which are beneficial to human health. Clitoria ternatea flower is a promising candidate for functional food applications owing to its wide range of pharmacotherapeutic properties as well as its safety and effectiveness.
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Adhikary R, Sultana S, Bishayi B (2018) Clitoria ternatea flower petals: effect on TNFR1 neutralization via downregulation of synovial matrix metalloproteases. J Ethnopharmacol 210:209–222. https://doi.org/10.1016/j.jep.2017.08.017
Admassu H, Gasmalla MA, Yang R et al (2018) Bioactive peptides derived from seaweed protein and their health benefits: antihypertensive, antioxidant, and antidiabetic properties. J Food Sci 83:6–16. https://doi.org/10.1111/1750-3841.14011
Alupului A, Calinescu I, Lavric V (2012) Microwave extraction of active principles from medicinal plants. UPB Sci Bull Ser B 74:129–142
Ambasta SP (1988) The Wealth of India: A Dictionary of India Raw Materials and Industrial Products, vol. II. Publication and Information Directorate, CSIR, New Delhi, India, p. 233. ISBN: 8185038902
Azima AS, Noriham A, Manshoor N (2017) Phenolics, antioxidants and color properties of aqueous pigmented plant extracts: Ardisia colorata var. elliptica, Clitoria ternatea, Garcinia mangostana and Syzygium cumini. J Funct Foods 38:232–241. https://doi.org/10.1016/j.jff.2017.09.018
Azmir J, Zaidul ISM, Rahman MM et al (2013) Techniques for extraction of bioactive compounds from plant materials: a review. J Food Eng 117:426–436. https://doi.org/10.1016/j.jfoodeng.2013.01.014
Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 6:71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Baskaran A, Mudalib SKA, Izirwan I (2019) Optimization of aqueous extraction of blue dye from butterfly pea flower. J Phys Conf Ser 1358:012001. https://doi.org/10.1088/1742-6596/1358/1/012001
Borikar SP, Kallewar NG, Mahapatra DK et al (2018) Dried flower powder combination of Clitoria ternatea and Punica granatum demonstrated analogous anti-hyperglycemic potential as compared with standard drug metformin: in vivo study in Sprague Dawley rats. J Appl Pharm Sci 8:75–79. https://doi.org/10.7324/japs.2018.81111
Brune K, Patrignani P (2015) New insights into the use of currently available non-steroidal anti-inflammatory drugs. J Pain Res 8:105. https://doi.org/10.2147/jpr.s75160
Cacace JE, Mazza G (2003) Mass transfer process during extraction of phenolic compounds from milled berries. J Food Eng 59:379–389. https://doi.org/10.1016/s0260-8774(02)00497-1
Caldas TW, Mazza KE, Teles AS et al (2018) Phenolic compounds recovery from grape skin using conventional and non-conventional extraction methods. Ind Crop Prod 111:86–91. https://doi.org/10.1016/j.indcrop.2017.10.012
Chauhan NS, Singh NK, Gupta JK et al (2017) A Review on Clitoria ternatea (Linn.): Chemistry and Pharmacology. Medicinal Plants and its Therapeutic Uses. OMICS Group eBooks, CA, USA. ISBN: 1632780747
Chaudhury A, Duvoor C, Dendi R et al (2017) Clinical review of antidiabetic drugs: implications for type 2 diabetes mellitus management. Front Endocrinol 8:6. https://doi.org/10.3389/fendo.2017.00006
Chauhan N, Rajvaidhya S, Dubey BK (2012) Pharmacognostical, phytochemical and pharmacological review on Clitoria ternatea for antiasthmatic activity. Int J Pharm Sci Res 3:398
Chayaratanasin P, Barbieri MA, Suanpairintr N et al (2015) Inhibitory effect of Clitoria ternatea flower petal extract on fructose-induced protein glycation and oxidation-dependent damages to albumin in vitro. BMC Complement Altern Med 15:27. https://doi.org/10.1186/s12906-015-0546-2
Chayaratanasin P, Caobi A, Suparpprom C et al (2019) Clitoria ternatea flower petal extract inhibits adipogenesis and lipid accumulation in 3T3-L1 preadipocytes by downregulating adipogenic gene expression. Molecules 24:1894. https://doi.org/10.3390/molecules24101894
Chong FC, Gwee XF (2015) Ultrasonic extraction of anthocyanin from Clitoria ternatea flowers using response surface methodology. Nat Prod Res 29:1485–1487. https://doi.org/10.1080/14786419.2015.1027892
Chusak C, Thilavech T, Henry CJ et al (2018) Acute effect of Clitoria ternatea flower beverage on glycemic response and antioxidant capacity in healthy subjects: a randomized crossover trial. BMC Complement Altern Med 18:1–11. https://doi.org/10.1186/s12906-017-2075-7
Chusak C, Ying JAY, Zhien JL et al (2019) Impact of Clitoria ternatea (butterfly pea) flower on in vitro starch digestibility, texture and sensory attributes of cooked rice using domestic cooking methods. Food Chem 295:646–652. https://doi.org/10.1016/j.foodchem
Corrales M, Toepfl S, Butz P et al (2008) Extraction of anthocyanins from grape by-products assisted by ultrasonics, high hydrostatic pressure or pulsed electric fields: a comparison. Innov Food Sci Emerg Technol 9:85–91. https://doi.org/10.1016/j.ifset.2007.06.002
Corrales M, García AF, Butz P et al (2009) Extraction of anthocyanins from grape skins assisted by high hydrostatic pressure. J Food Eng 90:415–421. https://doi.org/10.1016/j.jfoodeng.2008.07.003
Curigliano G, Cardinale D, Suter T et al (2012) Cardiovascular toxicity induced by chemotherapy, targeted agents and radiotherapy: ESMO Clinical Practice Guidelines. Ann Oncol 23:55–66. https://doi.org/10.1093/annonc/mds293
Daisy P, Rajathi M (2009) Hypoglycemic effects of Clitoria ternatea Linn. (Fabaceae) in alloxan-induced diabetes in rats. Trop J Pharm Res 8:393–398. https://doi.org/10.4314/tjpr.v8i5.48082
Escher GB, Marques MB, do Carmo MAV et al (2020) Clitoria ternatea L. petal bioactive compounds display antioxidant, antihemolytic and antihypertensive effects, inhibit α-amylase and α-glucosidase activities and reduce human LDL cholesterol and DNA induced oxidation. Food Res Int 128:108763. https://doi.org/10.1016/j.foodres.2019.108763
Fantz PR (1991) Ethnobotany of Clitoria (Leguminosae). Econ Bot 45:511–520. https://doi.org/10.1007/BF02930715
Gecer MK, Kan T, Gundogdu M et al (2020) Physicochemical characteristics of wild and cultivated apricots (Prunus armeniaca L.) from Aras valley in Turkey. Genet Resour Crop Eviron 67:935–945. https://doi.org/10.1007/s10722-020-00893-9
Gengatharan A, Dykes GA, Choo WS (2015) Betalains: natural plant pigments with potential application in functional foods. LWT-Food Sci Technol 64:645–649. https://doi.org/10.1016/j.lwt.2015.06.052
Havananda T, Luengwilai K (2019) Variation in floral antioxidant activities and phytochemical properties among butterfly pea (Clitoria ternatea L.) germplasm. Genet Resour Crop Eviron 66:645–658. https://doi.org/10.1007/s10722-018-00738-6
Herrera MC, De Castro ML (2005) Ultrasound-assisted extraction of phenolic compounds from strawberries prior to liquid chromatographic separation and photodiode array ultraviolet detection. J Chromatogr A 1100:1–7. https://doi.org/10.1016/j.chroma.2005.09.021
Iamsaard S, Burawat J, Kanla P et al (2014) Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats. J Zhejiang Univ Sci B 15:548–555. https://doi.org/10.1631/jzus.b1300299
Jain T (2009) Microwave assisted extraction for phytoconstituents: an overview. Asian J Chem 2:19–25
Jamil N, Zairi MNM, Nasim NAIM et al (2018) Influences of environmental conditions to phytoconstituents in Clitoria ternatea (butterfly pea flower): a review. J Sci Technol 10:208–228
Kamilla L, Mnsor SM, Ramanathan S et al (2009) Antimicrobial activity of Clitoria ternatea (L.) extracts. Pharmacologyonline 1:731–738
Kamkaen N, Wilkinson JM (2009) The antioxidant activity of Clitoria ternatea flower petal extracts and eye gel. Phytother Res 23:1624–1625. https://doi.org/10.1002/ptr.2832
Kazuma K, Noda N, Suzuki M (2003) Flavonoid composition related to petal color in different lines of Clitoria ternatea. Phytochemistry 64:1133–1139. https://doi.org/10.1016/s0031-9422(03)00504-1
Kosai P, Sirisidthi K, Jiraungkoorskul K et al (2015) Review on ethnomedicinal uses of memory boosting herb, butterfly pea, Clitoria ternatea. J Nat Remedies 15:71–76. https://doi.org/10.18311/jnr/2015/480
Kumar BS, Bhat KI (2011) In-vitro cytotoxic activity studies of Clitoria ternatea linn flower extracts. Int J Pharma Sci Rev Res 6:120–121
Lakshan SAT, Jayanath NY, Abeysekera WPKM et al (2019) A commercial potential blue pea (Clitoria ternatea L.) flower extract incorporated beverage having functional properties. Evid Based Complement Altern Med 2019:1–13. https://doi.org/10.1155/2019/2916914
Leong CR, Azizi K, Afif M et al (2017) Anthocyanins from Clitoria ternatea attenuate food-borne Penicillium expansum and its potential application as food biopreservative. Nat Prod Sci 23:125–131. https://doi.org/10.20307/nps.2017.23.2.125
López Prado AS, Shen Y, Ardoin R et al (2019) Effects of different solvents on total phenolic and total anthocyanin contents of Clitoria ternatea L. petal and their anti-cholesterol oxidation capabilities. Int J Food Sci Technol 54:424–431. https://doi.org/10.1111/ijfs.13953
Ludin AA, Al-Alwani MA, Mohamad AB et al (2018) Utilization of natural dyes from Zingiber officinale leaves and Clitoria ternatea flowers to prepare new photosensitisers for dye-sensitised solar cells. Int J Electrochem Sci 13(8):7451–7465. https://doi.org/10.20964/2018.08.04
Mahmad N, Taha RM, Othman R et al (2018) Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. Pigm Resin Technol 47:490–495. https://doi.org/10.1108/prt-11-2016-0109
Mathew N, Anitha MG, Bala TSL et al (2009) Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species. Parasitol Res 104:1017–1025. https://doi.org/10.1007/s00436-008-1284-x
Mauludifia F, Astrinia SD, Meiranti KA et al (2019) Production of natural colorant powder from Clitoria ternatea L. using tray dryer which is dehumidified by zeolite. J Phys Conf Ser 1295:012018
Mehmood A, Ishaq M, Zhao L et al (2019) Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). Ultrason Sonochem 51:12–19. https://doi.org/10.1016/j.ultsonch.2018.10.013
Mukherjee PK, Kumar V, Kumar NS et al (2008) The Ayurvedic medicine Clitoria ternatea-From traditional use to scientific assessment. J Ethnopharmacol 120:291–301. https://doi.org/10.1016/j.jep.2008.09.009
Nair V, Bang WY, Schreckinger E et al (2015) Protective role of ternatin anthocyanins and quercetin glycosides from butterfly pea (Clitoria ternatea Leguminosae) blue flower petals against lipopolysaccharide (LPS)-induced inflammation in macrophage cells. J Agric Food Chem 63:6355–6365. https://doi.org/10.1021/acs.jafc.5b00928
Neda GD, Rabeta MS, Ong MT (2013) Chemical composition and anti-proliferative properties of flowers of Clitoria ternatea. Int Food Res J 20:1229–1234
Nguyen GKT, Zhang S, Nguyen NTK et al (2011) Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family. J Biol Chem 286:24275–24287. https://doi.org/10.1074/jbc.m111.229922
Nirmal SA, Bhalke RD, Jadhav RS et al (2008) Anthelmintic activity of Clitoria ternatea. Pharmacologyonline 1:114–119
Nithianantham K, Ping KY, Latha LY et al (2013) Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage. Asian Pac J Trop Dis 3:314–319. https://doi.org/10.1016/s2222-1808(13)60075-4
Oguis GK, Gilding EK, Jackson MA et al (2019) Butterfly pea (Clitoria ternatea), a cyclotide-bearing plant with applications in agriculture and medicine. Front Plant Sci 10:645. https://doi.org/10.3389/fpls.2019.00645
Perfect JR (2016) Is there an emerging need for new antifungals? Expert Opin Emerg Drugs 21:129–131. https://doi.org/10.1517/14728214.2016.1155554
Pham TN, Nguyen DC, Lam TD et al (2019) Extraction of anthocyanins from Butterfly pea (Clitoria ternatea L. flowers) in Southern Vietnam: response surface modeling for optimization of the operation conditions. IOP Conf Ser Mater Sci Eng 542:012032. https://doi.org/10.1088/1757-899x/542/1/012032
Pham-Huy LA, He H, Pham-Huy C (2008) Free radicals, antioxidants in disease and health. Int J Biomed Sci 4:89–96
Phrueksanan W, Yibchok-anun S, Adisakwattana S (2014) Protection of Clitoria ternatea flower petal extract against free radical-induced hemolysis and oxidative damage in canine erythrocytes. Res Vet Sci 97:357–363. https://doi.org/10.1016/j.rvsc.2014.08.010
Rabeta MS, An Nabil Z (2013) Total phenolic compounds and scavenging activity in Clitoria ternatea and Vitex negundo linn. Int Food Res J 20:495–500
Rajamanickam M, Kalaivanan P, Sivagnanam I (2015) Evaluation of anti-oxidant and anti-diabetic activity of flower extract of Clitoria ternatea L. J Appl Pharm Sci 5:131–138. https://doi.org/10.7324/japs.2015.50820
Ranaganayaki S, Singh AK (1979) Isolation and identification of pigments of the flowers of Clitoria ternatea. J Indian Chem Soc 56:1037–1038
Reid R, Sinclair DF (1980) An evaluation of a collection of Clitoria ternatea for forage and grain production. CSIRO, Division of Tropical Crops & Pastures; 1980. ISSN: 01596071
Ruenroengklin N, Zhong J, Duan X et al (2008) Effects of various temperatures and pH values on the extraction yield of phenolics from litchi fruit pericarp tissue and the antioxidant activity of the extracted anthocyanins. Int J Mol 9:1333–1341. https://doi.org/10.3390/ijms9071333
Saito N, Abe K, Honda T et al (1985) Acylated delphinidin glucosides and flavonols from Clitoria ternatea. Phytochemistry 24:1583–1586
Scheffler RJ, Colmer S, Tynan H et al (2013) Antimicrobials, drug discovery, and genome mining. Appl Microbiol Biotechnol 97:969–978. https://doi.org/10.1007/s00253-012-4609-8
Senica M, Stampar F, Petkovsek MM (2019) Different extraction processes affect the metabolites in blue honeysuckle (Lonicera caerulea L. subsp. edulis) food products. Turk J Agric For 43:576–585. https://doi.org/10.3906/tar-1907-48
Senkal BC, Uskutoglu T, Cesur C et al (2019) Determination of essential oil components, mineral matter, and heavy metal content of Salvia virgata Jacq. grown in culture conditions. Turk J Agric For 43:395–404. https://doi.org/10.3906/tar-1812-84
Shen Y, Du L, Zeng H et al (2016) Butterfly pea (Clitoria ternatea) seed and petal extracts decreased Hep-2 carcinoma cell viability. Int J Food Sci Technol 51:1860–1868. https://doi.org/10.1111/ijfs.13158
Shyamkumar IB, Ishwar B (2012) Anti-inflammatory, analgesic and phytochemical studies of Clitoria ternatea Linn flower extract. Int Res J Pharm 3:208–210
Singh NK, Garabadu D, Sharma P et al (2018) Anti-allergy and anti-tussive activity of Clitoria ternatea L. in experimental animals. J Ethnopharmacol 224:15–26. https://doi.org/10.1016/j.jep.2018.05.026
Keka S, Saha PD, Ramya V et al (2012) Improved extraction of natural blue dye from butterfly pea using microwave assisted methodology to reduce the effect of synthetic blue dye. Int J Chem Technol 4:57–65. https://doi.org/10.3923/ijct.2012.57.65
Siti Azima AM, Noriham A, Manshoor N (2014) Anthocyanin content in relation to the antioxidant activity and colour properties of Garcinia mangostana peel, Syzigium cumini and Clitoria ternatea extracts. Int Food Res J 21:2369–2375
Sivaranjan VV, Balachandran I (1994) Ayurvedic drugs and their plant sources. Oxford & IBH Publishers Pvt., Ltd., New Delhi
Sreejith S, Samant MP, Jakhar JK et al (2014) Modeling the impact of extraction conditions on functional properties of gelatin from scales of blackspotted croaker (Protonibea diacanthus). Proc Natl A Sci India B 84:1021–1029. https://doi.org/10.1007/s40011-013-0259-6
Srichaikul B (2018) Ultrasonication extraction, bioactivity, antioxidant activity, total flavonoid, total phenolic and antioxidant of Clitoria ternatea linn flower extract for anti-aging drinks. Pharmacogn Mag 14:322. https://doi.org/10.4103/pm.pm_206_17
Subramanian MS, Prathyusha P (2011) Pharmaco-phytochemical characterization of Clitoria ternatea Linn. Int J Pharmtech Res 3:606–612
Terahara N, Saito N, Honda T et al (1989) Structure of ternatin D1, an acylated anthocyanin from Clitoria ternatea flowers. Tetrahedron Lett 30:5305–5308. https://doi.org/10.1016/s0040-4039(01)93771-2
Terahara N, Oda M, Matsui T et al (1996) Five new anthocyanins, ternatins A3, B4, B3, B2, and D2, from Clitoria ternatea flowers. J Nat 59:139–144. https://doi.org/10.1021/np960050a
Terahara N, Toki K, Saito N et al (1998) Eight new anthocyanins, ternatins C1–C5 and D3 and preternatins A3 and C4 from young Clitoria ternatea flowers. J Nat 61:1361–1367. https://doi.org/10.1021/np980160c
Uma B, Prabhakar K, Rajendran S (2009) Phytochemical analysis and antimicrobial activity of Clitorea ternatea Linn against extended spectrum beta lactamase producing enteric and urinary pathogens. Asian J Pharm Clin Res 2:94–96
Wen C, Zhang J, Zhang H et al (2018) Advances in ultrasound assisted extraction of bioactive compounds from cash crops: a review. Ultrason Sonochem 48:538–549. https://doi.org/10.1016/j.ultsonch.2018.07.018
Zakaria NNA, Okello EJ, Howes MJ et al (2018) In vitro protective effects of an aqueous extract of Clitoria ternatea L. flower against hydrogen peroxide-induced cytotoxicity and UV-induced mtDNA damage in human keratinocytes. Phytother Res 32:1064–1072. https://doi.org/10.1002/ptr.6045
Zhang Z, Pang X, Ji Z et al (2001) Role of anthocyanin degradation in litchi pericarp browning. Food Chem 75:217–221. https://doi.org/10.1016/s0308-8146(01)00202-3
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This work was funded by the School of Science, Monash University Malaysia.
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Jeyaraj, E.J., Lim, Y.Y. & Choo, W.S. Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals. J Food Sci Technol 58, 2054–2067 (2021). https://doi.org/10.1007/s13197-020-04745-3
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DOI: https://doi.org/10.1007/s13197-020-04745-3