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Pro-oxidant versus anti-oxidant effects of seeds aglycone extracts of Lepidium sativum and Eruca vesicaria Linn., in vitro, and on neutrophil nitro-oxidative functions

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Abstract

This study evaluated the anti-inflammatory and antioxidant properties of seeds aglycone extracts from Lepidium sativum (LS) and Eruca vesicaria (EV) Linn., on oxidative damages in vitro and on neutrophil nitro-oxidative functions. The results showed that LS and EV aglycone extracts attenuated liver microsomal lipids and proteins oxidation through a potent antioxidant effect as attested by the dose dependent quenching of DPPH radical scavenging activity. LS and EV aglycone extracts inhibited dose dependently the production of superoxide anion by BALB/c mice-derived peritoneal neutrophils, whereas they slightly enhanced exocytosis of myeloperoxidase (MPO), a marker of azurophilic granules. Interestingly, only LS replenished glutathione (GSH) and nitric oxide levels, indicating a fine differential effect. This study highlighted the subtle oxidative and antioxidant capacity of LS and EV seeds aglycone extracts. These health promoting compounds could be used to finely modulate critical events involved in microbial infection, inflammation and nitro-oxidative stress.

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Abbreviations

AA:

Ascorbic acid

EV:

Eruca vesicaria

GSH:

Reduced glutathione

HNE:

4-Hydroxynonenal

LPI:

Lipid peroxidation inhibition

LS:

Lepidium sativum

MDA:

Malondialdehyde

MPO:

Myeloperoxidase

NAC:

N-Acetylcysteine

NO:

Nitric oxide

NOS:

Nitric oxide synthase

NOX2:

NADPH oxidase2

ProtCO:

Protein carbonyls

PN:

Neutrophils

ROS:

Reactive oxygen species

O ·−2 :

Superoxide anion

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Acknowledgements

The authors are grateful to Dr Haj-Arab. H. for the identification of the plant

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Authors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology, Houari Boumediene, BP 32, El-Alia, Bab-Ezzouar, 16111, Algiers, Algeria

    Nabila Tounsi & Bahia Djerdjouri

  2. Laboratory of Plant Physiology, Faculty of Biological Sciences, University of Sciences and Technology, Houari Boumediene, Algiers, Algeria

    Ouahiba Ait Yahia & Aicha Belkebir

Authors
  1. Nabila Tounsi
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  2. Bahia Djerdjouri
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  3. Ouahiba Ait Yahia
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  4. Aicha Belkebir
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Correspondence to Bahia Djerdjouri.

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Tounsi, N., Djerdjouri, B., Yahia, O.A. et al. Pro-oxidant versus anti-oxidant effects of seeds aglycone extracts of Lepidium sativum and Eruca vesicaria Linn., in vitro, and on neutrophil nitro-oxidative functions. J Food Sci Technol 56, 5492–5499 (2019). https://doi.org/10.1007/s13197-019-04021-z

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  • DOI: https://doi.org/10.1007/s13197-019-04021-z

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