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Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent

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Abstract

Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 ± 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 ± 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 μg/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.

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Acknowledegement

This research was supported by the Ministry of Education, Science and Technological Development of Republic of Serbia (Grants Nos. TR 31089 and ON 173021).

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Authors and Affiliations

  1. Clinical Centre of Vojvodina, Biochemistry Department, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, 21000, Serbia

    Tatjana Ćebović

  2. Department of Pharmacognosy, University of Belgrade - Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, 11221, Serbia

    Jelena Arsenijević, Milica Drobac & Zoran Maksimović

  3. Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, Belgrade, 11000, Serbia

    Jelena Živković

  4. Department of Crop Science, University of Belgrade - Faculty of Agriculture, Nemanjina 6, Zemun, Belgrade, 11080, Serbia

    Ivan Šoštarić

Authors
  1. Tatjana Ćebović
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  2. Jelena Arsenijević
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  3. Milica Drobac
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  4. Jelena Živković
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  5. Ivan Šoštarić
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  6. Zoran Maksimović
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Correspondence to Jelena Arsenijević.

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Ćebović, T., Arsenijević, J., Drobac, M. et al. Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. J Food Sci Technol 55, 560–567 (2018). https://doi.org/10.1007/s13197-017-2965-1

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  • DOI: https://doi.org/10.1007/s13197-017-2965-1

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