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Quantification of phenolics in Syzygium cumini seed and their modulatory role on tertiary butyl-hydrogen peroxide-induced oxidative stress in H9c2 cell lines and key enzymes in cardioprotection

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Abstract

The study quantified the major phenolics in different fractions of Syzygium cumini seeds and evaluated their cardioprotective efficacy. Gallic acid, ellagic acid, cinnamic acid, quercetin, syringic acid and ferulic acid were the major polyphenols present in different fractions of Syzygium cumini seeds. The cardioprotective effect of Syzygium cumini seed fractions in modulating angiotensin converting enzyme (ACE), HMG-CoA reductase, LDL oxidation and tertiary butyl hydrogen peroxide (TBHP) induced oxidative stress in H9c2 cardiac cell lines were investigated. Syzygium cumini effectively attenuated the cellular oxidative stress in H9c2 cardiomyoblasts. These fractions possessed inhibitory potential against ACE, HMG-CoA reductase and LDL oxidation. Molecular docking studies of the predominant polyphenols with ACE and HMG-CoA proteins revealed the binding interactions of these compounds, thus confirming their modulation of activity. The present study demonstrated the cardioprotective efficacy of Syzygium cumini seed fractions which can be attributed to the presence of phenolic acids and flavonoids.

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References

  • Atale N, Chakraborty M, Mohanty S, Bhattacharya S, Nigam D, Sharma M, Rani V (2013) Cardioprotective role of Syzygium cumini against glucose-induced oxidative stress in H9c2 cardiac myocytes. Cardiovasc Toxicol 13(3):278–289

    Article  CAS  Google Scholar 

  • Ayyanar M, Subash-Babu P (2012) Syzygium cumini (L.) Skeels: a review of its phytochemical constituents and traditional uses. Asian Pac J Trop Biomed 2(3):240–246. doi:10.1016/S2221-1691(12)60050-1

    Article  Google Scholar 

  • Ayyanar M, Subash-Babu P, Ignacimuthu S (2013) Syzygium cumini (L.) Skeels a novel therapeutic agent for diabetes: folk medicinal and pharmacological evidences. Complement Ther Med 21(3):232–243. doi:10.1016/j.ctim.2013.03.004

    Article  Google Scholar 

  • Baba S, Osakabe N, Kato Y, Natsume M, Yasuda A, Kido T, Kondo K (2007) Continuous intake of polyphenolic compounds containing cocoa powder reduces LDL oxidative susceptibility and has beneficial effects on plasma HDL-cholesterol concentrations in humans. Am J Clin Nutr 85(3):709–717

    CAS  Google Scholar 

  • Baliga MS, Bhat HP, Baliga BRV, Wilson R, Palatty PL (2011) Phytochemistry, traditional uses and pharmacology of Eugenia jambolana Lam. (black plum): a review. Food Res Int 44(7):1776–1789. doi:10.1016/j.foodres.2011.02.007F

    Article  CAS  Google Scholar 

  • Chen H, Zuo YG, Deng YW (2001) Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. J Chromatogr A 913(1–2):387–395. doi:10.1016/S0021-9673(00)01030-X

    Article  CAS  Google Scholar 

  • Chidambara Murthy KN, Singh RP, Jayaprakasha GK (2002) Antioxidant activities of grape (Vitis vinifera) pomace fractions. J Agric Food Chem 50(21):5909–5914

    Article  Google Scholar 

  • Habauzit V, Morand C (2012) Evidence for a protective effect of polyphenols-containing foods on cardiovascular health: an update for clinicians. Ther Adv Chronic Dis 3(2):87–106

    Article  CAS  Google Scholar 

  • Itabe H, Obama T, Kato R (2011) The dynamics of oxidized LDL during atherogenesis. J Lipids. doi:10.1155/2011/418313

    Google Scholar 

  • Kumar R, Kumar A, Sharma R, Baruwa A (2010) Pharmacological review on natural ACE inhibitors. Der Pharmacia Lettre 2(2):273–293

    Google Scholar 

  • Kuo MY, Ou HC, Lee WJ, Kuo WW, Hwang LL, Song TY, Sheu WH (2011) Ellagic acid inhibits oxidized low-density lipoprotein (OxLDL)-induced metalloproteinase (MMP) expression by modulating the protein kinase C-alpha/extracellular signal-regulated kinase/peroxisome proliferator-activated receptor gamma/nuclear factor-kappaB (PKC-alpha/ERK/PPAR-gamma/NF-kappaB) signaling pathway in endothelial cells. J Agric Food Chem 59(9):5100–5108. doi:10.1021/jf1041867

    Article  CAS  Google Scholar 

  • LeBel CP, Ischiropoulos H, Bondy SC (1992) Evaluation of the probe 2′,7′-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem Res Toxicol 5(2):227–231

    Article  CAS  Google Scholar 

  • Lloyd-Jones D, Adams RJ, Brown TM, Carnethon M, Dai S, De Simone G, Ferguson TB, Ford E, Furie K, Gillespie C, Go A (2010) Heart disease and stroke statistics—2010 update. Circulation 121(7):e46–e215

    Article  Google Scholar 

  • Mahindroo N, Wang CC, Liao CC, Huang CF, Lu IL, Lien TW, Hsieh HP (2006) Indol-1-yl acetic acids as peroxisome proliferator-activated receptor agonists: design, synthesis, structural biology, and molecular docking studies. J Med Chem 49(3):1212–1216. doi:10.1021/jm0510373

    Article  CAS  Google Scholar 

  • Morris GM, Huey R, Lindstrom W, Sanner MF, Belew RK, Goodsell DS, Olson AJ (2009) AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility. J Comput Chem 30(16):2785–2791. doi:10.1002/jcc.21256

    Article  CAS  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63. doi:10.1016/0022-1759(83)90303-4

    Article  CAS  Google Scholar 

  • Mozaffarian D, Benjamin EJ, Go AS, Arnett DK, Blaha MJ, Cushman M, Subcomm SS (2015) Heart disease and stroke statistics-2015 update a report from the american heart association. Circulation 131(4):E29–E322. doi:10.1161/Cir.0000000000000152

    Article  Google Scholar 

  • Paiva LBd, Rosana G, Santos WD, Squina FMS (2013) Ferulic acid and derivatives: molecules with potential application in the pharmaceutical field. Braz J Pharm Sci 49(3):395–411

    Article  Google Scholar 

  • Pandey KB, Rizvi SI (2009) Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2(5):270–278

    Article  Google Scholar 

  • Pashkow FJ (2011) Oxidative stress and inflammation in heart disease: do antioxidants have a role in treatment and/or prevention? Int J Inflam 11:2011

    Google Scholar 

  • Patel Soncharan STS, Somasundaram I, Niladri Maity (2010) Protective effect of Syzygium cumini seeds against doxorubicin induced cardiotoxicity in rats. Int J Pharm Life Sci 1(6):343–349

    Google Scholar 

  • Priya SH, Prakasan N, Purushothaman J (2017) Antioxidant activity, phenolic-flavonoid content and high-performance liquid chromatography profiling of three different variants of Syzygium cumini seeds: a comparative study. J Intercult Ethnopharmacol 6(1):107

    Article  Google Scholar 

  • Rahman T, Hosen I, Towhidul Islam MM, Shekhar HU (2012) Oxidative stress and human health. Adv Biosci Biotechnol 3:997–1019

    Article  Google Scholar 

  • Safari MR, Rezaie M, Sheikh N (2003) Efects of some flavonoids on the susceptibility of low-density lipoprotein to oxidative modification. Indian J Biochem Biophys 40(5):358–361

    CAS  Google Scholar 

  • Santhosh M, Ramarethinam S, Latha K (2008) Comparative analysis of HMG CoA inhibitors via computational tools. JCIB 1:119–128

    Google Scholar 

  • Saroj A, Pragadheesh VS, Palanivelu Yadav A, Singh SC, Samad A, Chanotiya CS (2015) Anti-phytopathogenic activity of Syzygium cumini essential oil, hydrocarbon fractions and its novel constituents. Ind Crops Prod 74:327–335. doi:10.1016/j.indcrop.2015.04.065

    Article  CAS  Google Scholar 

  • Schroeder N, Park YH, Kang MS, Kim Y, Ha GK, Kim HR, Caballero B (2015) A randomized trial on the effects of 2010 dietary guidelines for Americans and Korean diet patterns on cardiovascular risk factors in overweight and obese adults. J Acad Nutr Diet 115(7):1083–1092. doi:10.1016/j.jand.2015.03.023

    Article  Google Scholar 

  • The PyMOL Molecular Graphics System (2015) (Version 1.8 Schrödinger, LLC)

  • Varsha Tegeli PK (2014) Importance of free radical and antioxidant on human health. Int J Pharma Chem Biolo Sci 4(4):1038–1050

    Google Scholar 

  • Wijesekara I, Kim SK (2010) Angiotensin-I-converting enzyme (ACE) inhibitors from marine resources: prospects in the pharmaceutical industry. Mar Drugs 8(4):1080–1093

    Article  CAS  Google Scholar 

  • Yang Y, Chan SW, Hu M, Walden R, Tomlinson B (2011) Effects of some common food constituents on cardiovascular disease. ISRN cardiol 16:2011

    Google Scholar 

  • Zafar R (2015) A new insight into pathogenesis of cardiovascular diseases: stress induced lipid mediated, vascular diseases. J Cardiovasc Dis Diagn 3:197. doi:10.4172/2329-9517.1000197

    Google Scholar 

Download references

Acknowledgements

The authors are thankful to Department of Science and Technology (INSPIRE), India, and Council for Scientific and Industrial Research, India for the financial support.

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Authors and Affiliations

  1. Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram, 695019, Kerala, India

    H. P. Syama, R. Dhanya, P. Nisha, A. Sundaresan & P. Jayamurthy

  2. Computational Modeling and Simulation, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram, 695019, Kerala, India

    A. D. Arya & E. Jacob

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  1. H. P. Syama
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  2. A. D. Arya
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  3. R. Dhanya
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  4. P. Nisha
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Correspondence to P. Jayamurthy.

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Syama, H.P., Arya, A.D., Dhanya, R. et al. Quantification of phenolics in Syzygium cumini seed and their modulatory role on tertiary butyl-hydrogen peroxide-induced oxidative stress in H9c2 cell lines and key enzymes in cardioprotection. J Food Sci Technol 54, 2115–2125 (2017). https://doi.org/10.1007/s13197-017-2651-3

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  • DOI: https://doi.org/10.1007/s13197-017-2651-3

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