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Quantitative determination of melatonin in milk by LC-MS/MS

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Abstract

A rapid, reliable and sensitive LC-MS/MS method for the determination of melatonin in milk was developed and validated. Sample was extracted with dichloromethane and cleaned by passing through Chem Elut solid phase extraction cartridge. The solvent was evaporated to dryness, reconstituted with methanol and analysed by LC-MS/MS on Agilent zorbax Eclipse XDB C-18 rapid resolution analytical column. The analytical procedure was found to be accurate, precise and linear. The method accuracy was 92.2 % (range 90.06–94.58) and the mean precision was 1.55 % and the calibration was linear for 1 to 150 pg mL−1 (R2 > 0.99), the lowest limit of quantification (LLOQ) was 1 pg mL−1. 7-D Melatonin (7-DM) was used as an internal standard. This method was proved to be a promising method for the determination of melatonin for market milk and human milk samples.

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Authors and Affiliations

  1. Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamilnadu, 620 015, India

    Duraisamy Karunanithi & Valsala Madhavan Nair Biju

  2. Analytical Science Division, Shriram Institute for Industrial Research, Bangalore, Karnataka, 560 048, India

    Duraisamy Karunanithi, Ammanamanchi Radhakrishna & Kunnummal Parambil Sivaraman

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  1. Duraisamy Karunanithi
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  2. Ammanamanchi Radhakrishna
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  3. Kunnummal Parambil Sivaraman
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  4. Valsala Madhavan Nair Biju
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Correspondence to Valsala Madhavan Nair Biju.

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Karunanithi, D., Radhakrishna, A., Sivaraman, K.P. et al. Quantitative determination of melatonin in milk by LC-MS/MS. J Food Sci Technol 51, 805–812 (2014). https://doi.org/10.1007/s13197-013-1221-6

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  • DOI: https://doi.org/10.1007/s13197-013-1221-6

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