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Separation and quantification of neoagaro-oligosaccharides

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Abstract

Oligosaccharides were obtained from agar by enzymatic hydrolysis. Activated carbon adsorption separation was used to extract oligosaccharides, and gel chromatography separation was applied to further purify oligosaccharides. The result showed that activated carbon adsorption could remove the most salt impurities, and gel column chromatography could give the separation of the two kinds of oligosaccharides. ESI-MS, 13C-NMR revealed that the molecular weight (Mw) of two oligosaccharides were 630 and 936, which were identified as neoagarotetraose and neoagarohexaose respectively.

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Acknowledgements

This work was supported by the financial support from the Science and Technology Department of Zhejiang Province (2009C14027)

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Authors and Affiliations

  1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, China

    Jianfeng Mei, Jinting Shao, Hong Wang, Yu Yi & Guoqing Ying

  2. Food Research Program, Agriculture and Agri-Food Canada, Guelph, N1G 5C9, ON, Canada

    Qi Wang

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  1. Jianfeng Mei
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  2. Jinting Shao
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  3. Qi Wang
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  4. Hong Wang
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  5. Yu Yi
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Correspondence to Guoqing Ying.

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Mei, J., Shao, J., Wang, Q. et al. Separation and quantification of neoagaro-oligosaccharides. J Food Sci Technol 50, 1217–1221 (2013). https://doi.org/10.1007/s13197-011-0448-3

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  • DOI: https://doi.org/10.1007/s13197-011-0448-3

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