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The separate and combined influence of antioxidant and anti-inflammatory drugs on the liver during ischaemia-reperfusion of the superior mesenteric artery: An experimental study

  • Experimental Study
  • Published:
Hellenic Journal of Surgery

Abstract

Aim-Background

To investigate the influence of N-acetyl-cysteine (NAC) and Atorvastatin, separately and combined, on the liver of rats after inducing ischaemia-reperfusion of the superior mesenteric artery.

Methods

Forty male rats aged 2½ months and weighing 220–250gr were randomly assigned to 5 groups of 8 rats. The rats in the first group were considered as controls. The second group was subjected to ischaemia of the superior mesenteric artery for 45 minutes, followed by reperfusion of the superior mesenteric artery for 3 hours, without any drug administration. In the third group, a single dose of Atorvastatin (10mg/kg) was administered 24 hours before the phase of ischaemia. The fourth group received a single dose of N-acetyl-cysteine (160mg/kg) 24 hours before the phase of ischaemia. The fifth group was given both drugs simultaneously 24 hours before the phase of ischaemia.

Three hours after reperfusion of the superior mesenteric artery, the liver was surgically removed and prepared for histological examination.

Results

The histological examination of liver tissue samples revealed diffuse necrosis of hepatocytes in the group without any drug administration prior to ischaemia-reperfusion. The groups of animals that were treated with NAC or Atorvastatin or the combination of both drugs showed quite a different histological picture with focal necrosis of hepatocytes and less degenerative lesions.

Conclusions

This study shows that pretreatment with NAC, Atorvastatin and their combination protects the liver from injury induced by intestinal ischaemia-reperfusion. This is ascribed to the antioxidant and anti-inflammatory effect of the above-mentioned drugs.

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Authors and Affiliations

  1. Department of Vascular Surgery G.H., “G.Gennimatas”, Athens, Greece

    D. Alexandropoulos

  2. Department of Vascular Surgery G.H., “Sismanogleio”, Athens, Greece

    G. Bazigos

  3. Department of Cardiology G.H., “Agios Andreas”, Patra, Greece

    N. Tragotsalou

  4. 2nd Clinic of Surgery, Aretaieion Hospital, University of Athens, Athens, Greece

    T. Kotsis

  5. 4th General Surgery Department, Attikon Hospital, University of Athens, Athens, Greece

    V. Smyrniotis

  6. Experimental Surgery and Surgical Research N.S Christeas, University of Athens, Athens, Greece

    D. Perrea

  7. Laboratory of Experimental Surgery and Surgical Research N.S Christeas, University of Athens, Athens, Greece

    A. Pantopoulou

  8. Pathology Laboratory, Aretaieion University Hospital Athens, Athens, Greece

    A. Kondi-Pafiti

  9. Menippou 30A Holargos, Athens, Greece

    D. Alexandropoulos

Authors
  1. D. Alexandropoulos
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  2. G. Bazigos
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  3. N. Tragotsalou
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  4. T. Kotsis
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  5. V. Smyrniotis
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  6. D. Perrea
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Correspondence to D. Alexandropoulos.

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Alexandropoulos, D., Bazigos, G., Tragotsalou, N. et al. The separate and combined influence of antioxidant and anti-inflammatory drugs on the liver during ischaemia-reperfusion of the superior mesenteric artery: An experimental study. Hellenic J Surg 85, 254–260 (2013). https://doi.org/10.1007/s13126-013-0049-x

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  • DOI: https://doi.org/10.1007/s13126-013-0049-x

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