Abstract
The current study was undertaken to determine the effect of the troglitazone (TGZ) on the expression of peroxisome proliferator-activating receptor (PPARγ), CCAAT/enhancer-binding protein, fatty acid binding protein 4, calpain 1 (CAPN1), and lipid accumulation in the myotube of Hanwoo muscle satellite cells. The satellite cells were treated with 5, 10, and 50 μM of TGZ for indicated time intervals. TGZ promoted the trans-differentiation with significant increase in glycerol accumulation. Polymerase chain reaction (PCR) and microarray results indicated that the TGZ treatment significantly increased the expression of adipogenic transcription factors. TGZ (10 and 50 μM) increased the CAPN1 gene expression 2.2- and 2.6-fold in real-time polymerase chain reaction analysis and 0.52- and 0.25-fold in microarray analysis, respectively, when compared with their respective controls. This result suggests that CAPN1 gene might be involved in the adipogenic differentiation programs. In addition, 13 genes were upregulated and 12 genes were downregulated in microarray analysis. Most of the up/downregulated genes were directly linked with adipogenesis.
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Abbreviations
- TGZ:
-
Troglitazone
- CEBPα:
-
CCAAT/enhancer-binding protein alpha
- CEBPβ:
-
CCAAT/enhancer-binding protein beta
- FABP4:
-
Fatty acid binding protein 4
- PPARγ:
-
Peroxisome proliferator-activating receptor
- CAPN1:
-
Calpain 1
- TZD:
-
Thiazolidinedioes
- PGZ:
-
Pioglitazone
- RGZ:
-
Rosiglitazone
- PCR:
-
Polymerase chain reaction
- RT-PCR:
-
Real-time polymerase chain reaction
- FBS:
-
Fetal bovine serum
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Acknowledgments
It should be acknowledged that this work was supported by the research grants for the Next Generation Biogreen Projects (no. PJ008191), RDA and the research grants for the FTA issue project (no. PJ907055), RDA, Republic of Korea.
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Yang, Y.B., Pandurangan, M., Jeong, D. et al. The effect of troglitazone on lipid accumulation and related gene expression in Hanwoo muscle satellite cell. J Physiol Biochem 69, 97–109 (2013). https://doi.org/10.1007/s13105-012-0193-8
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DOI: https://doi.org/10.1007/s13105-012-0193-8