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Brain Alpha- and Beta-Globin Expression after Intracerebral Hemorrhage

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Abstract

Our recent study has demonstrated that hemoglobin (Hb) is present in cerebral neurons, and neuronal Hb is inducible after cerebral ischemia. In the present study, we examined the effects of intracerebral hemorrhage (ICH) on the messenger RNA (mRNA) levels of the α-globin (HbA) and the β-globin (HbB) components of Hb and Hb protein in the brain in vivo and in vitro. In vivo, male Sprague–Dawley rats received either a needle insertion (sham) or an infusion of autologous whole blood into the basal ganglia and were killed at different time points. In vitro, cultured rat brain cells were used for HbA, HbB, and Hb determination. Cultured neurons were exposed to 50 or 100 µM hemin for 24 h. Some neurons also were treated with deferoxamine, an iron chelator, or vehicle. Levels of HbA and HbB, Hb, and hemopexin, a transporter of heme, were measured. We found that HbA, HbB, and Hb are primarily expressed in neurons, with much lower expression in astrocytes and microglia. HbA, HbB, and Hb expression in the perihematomal zone was increased after ICH and Hb was localized in neurons and glia. Hemin increased HbA, HbB, and hemopexin mRNA levels in cultured neurons. Deferoxamine reduced hemin-induced neuronal Hb expression. ICH increased HbA and HbB expression in the brain, which may potentially serve to buffer the heme released during clot resolution.

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Acknowledgments

This study was supported by grants NS-017760, NS-039866, NS-047245 NS-052510, and NS-057539 from the National Institutes of Health (NIH) and 0755717Z and 0840016N from American Heart Association (AHA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and AHA. MMW is supported by NS-062816 and NS-054724.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Michigan, R5018 Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200, USA

    Yangdong He, Ya Hua, Jin-Yul Lee, Wenquan Liu, Richard F. Keep & Guohua Xi

  2. Department of Neurology, University of Michigan, R5018 Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, MI, USA

    Michael M. Wang

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  1. Yangdong He
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  2. Ya Hua
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  3. Jin-Yul Lee
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  4. Wenquan Liu
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  5. Richard F. Keep
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  6. Michael M. Wang
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  7. Guohua Xi
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Correspondence to Guohua Xi.

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He, Y., Hua, Y., Lee, JY. et al. Brain Alpha- and Beta-Globin Expression after Intracerebral Hemorrhage. Transl. Stroke Res. 1, 48–56 (2010). https://doi.org/10.1007/s12975-009-0004-x

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  • DOI: https://doi.org/10.1007/s12975-009-0004-x

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