Abstract
Salweenia bouffordiana is an endangered species of Qinghai-Tibetan Plateau (QTP) in China. In this study, its complete plastome was assembled using next-generation sequencing. The complete genome is 153, 730 bp in length, comprises a pair of inverted repeat (IR; 26, 227 bp) regions separated by the large single-copy (LSC; 83, 509 bp) and small single-copy (SSC; 17, 767 bp) regions. It encodes 111 genes, including 77 protein-coding genes (PCGs), 30 tRNAs and 4 rRNAs. The nucleotide composition is asymmetric (31.6% A, 18.1% C, 18.7% G and 31.6% T) with an overall A+T content of 73.2%. Three types of 111 simple sequence repeats (SSRs) were detected in the plastome, including 107 mono- and 4 di-nucleotide repeats. The newly sequenced complete plastome and identified SSRs will help in understanding the plastome evolution and genetic conservation of S. bouffordiana.
References
Baker EG (1935) New genus of Leguminosae from Tibet. J Bot 73:134–136
Ding CS (1994) Robinieae. In: Wei Z (ed) Flora Reipublicae Popularis Sinicae, vol 40. Science Press, Beijing, pp 226–228
IUCN (2012). IUCN Red List Categories and Criteria, version 3.2. Prepared by the IUCN Species Survival Commission. IUCN, Gland
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierern T, Ashton B, Meintjes P, Dremmond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649
Langmead B, Salzberg SL (2012) Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357–359
Li PC, Ni ZC (1982) The formation and differentiation of the Leguminosae flora in Xizang (Tibet). Acta Phytotaxon Sin 20:142–156
Lohse M, Drechsel O, Kahlau S, Bock R (2013) OrganellarGenomeDRAW-a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res 41:W575–W581
Mukherjee A, Williams D, Gitzendanner MA, Overholt WA, Cuda JP (2016) Microsatellite and chloroplast DNA diversity of the invasive aquatic weed Hygrophila polysperma in native and invasive ranges. Aquat Bot 129:55–61. doi:10.1016/j.aquabot.2015.12.004
Tillich M, Lehwark P, Pellizzer T, Ulbricht-Jones ES, Fischer A, Bock R, Greiner S (2017) GeSeq-versatile and accurate annotation of organelle genomes. Nucleic Acids Res doi:10.1093/nar/gkx391
Twyford AD, Ness RW (2017) Strategies for complete plastid genome sequencing. Mol Ecol Resour. doi:10.1111/1755-0998.12626
Wang HS (1989) A study on the origin of spermatophytic genera endemic to China. Acta Bot Yunnan 11:1–16
Wick RR, Schultz MB, Zobel J, Holt KE (2015) Bandage: interactive visualization of de novo genome assemblies. Bioinformatics 31:3350–3352
Wu ZY, Lu AM, Tang YC, Chen ZD, Li DZ (2003) The families and genera of angiosperms in China: a comprehensive analysis. Science Press, Beijing
Yue XK, Yue JP, Yang LE, Li ZM, Sun H (2011) Systematics of the genus Salweenia (Leguminosae) from Southwest China with discovery of a second species. Taxon 60:1366–1374
Acknowledgements
We appreciate funding from the Talent Project of Yunnan Province (No. 2011CI042) and are grateful to the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences for providing DNA materials.
Funding
This study was funded by grant from the Talent Project of Yunnan Province (No. 2011CI042).
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Wariss, H.M., Yi, TS., Wang, H. et al. The chloroplast genome of a rare and an endangered species Salweenia bouffordiana (Leguminosae) in China. Conservation Genet Resour 10, 405–407 (2018). https://doi.org/10.1007/s12686-017-0836-8
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DOI: https://doi.org/10.1007/s12686-017-0836-8