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Development and characterization of polymorphic microsatellite markers for the crested caracara, Caracara cheriway

  • Microsatellite Letters
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Abstract

We isolated novel microsatellites from the crested caracara (Caracara cheriway) with a shotgun pyrosequencing approach. We tested 80 loci for polymorphism among 20 individuals from the threatened Florida population. Fourteen loci were polymorphic. The mean number of alleles was 2.21 (range 2–3) and the mean observed heterozygosity was 0.41 (range 0.15–0.65). None of the 14 polymorphic loci exhibited significant linkage disequilibrium nor did they deviate significantly from Hardy–Weinberg expectations. We also report 16 monomorphic loci.

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References

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Acknowledgments

We would like to thank Debbie Chen and Mila Crevar for their assistance in primer testing. Any use of trade, firm, or product names is for descriptive purpose only and does not imply endorsement by the US Government.

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Authors and Affiliations

  1. Genetics Graduate Interdisciplinary Program, University of Arizona, Tucson, AZ, 85721, USA

    Erin E. Vaughn

  2. Conservation Genetics Laboratory, School of Natural Resources and Environment, University of Arizona, BSE-325, Tucson, AZ, 85721, USA

    Erin E. Vaughn & Melanie Culver

  3. Department of Biology, Trinity College, 300 Summit St., Hartford, CT, 06106, USA

    Joan L. Morrison

  4. US Geological Survey, Arizona Cooperative Fish and Wildlife Research Unit, Tucson, AZ, USA

    Melanie Culver

  5. EDM International, Inc., Fort Collins, CO, 80525, USA

    James F. Dwyer

Authors
  1. Erin E. Vaughn
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  2. James F. Dwyer
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  3. Joan L. Morrison
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  4. Melanie Culver
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Corresponding author

Correspondence to Erin E. Vaughn.

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Vaughn, E.E., Dwyer, J.F., Morrison, J.L. et al. Development and characterization of polymorphic microsatellite markers for the crested caracara, Caracara cheriway . Conservation Genet Resour 7, 557–559 (2015). https://doi.org/10.1007/s12686-015-0423-9

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  • DOI: https://doi.org/10.1007/s12686-015-0423-9

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