Abstract
We characterized nine polymorphic microsatellites by an enriched library from the Antarctic silverfish Pleuragramma antarcticum, a key species in the high Antarctic zone of the Southern Ocean. The number of alleles scored ranged from 7 to 39, whereas the observed and expected heterozygosity ranged from 0.4000 to 0.9750 and from 0.3943 to 0.9782, respectively. Cross-amplification was tested in the 3 species of the genus Chionodraco (Channichthyidae). These new microsatellites could potentially be useful in further investigations on P. antarcticum for which many questions on population structure and exposition to global environment change are still open to debate.
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Acknowledgements
This work was supported by the Italian Antarctic Research Programme (PNRA) and by National Science Foundation (award number 0741348). We thank Prof. S. Greco for samples collection during the 5th Italian expedition, PNRA, V. Varotto for samples collection during the 11th and 13th Italian expeditions, PNRA and Dr. K.H. Kock and AWI (Alfred-Wegener-Institute für Polar und Meeresforschung) who collected the 1996 sample during the ANT-XIV/2 Polarstern Cruise.
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Papetti, C., Marino, I.A.M., Agostini, C. et al. Characterization of novel microsatellite markers in the Antarctic silverfish Pleuragramma antarcticum and cross species amplification in other Notothenioidei. Conservation Genet Resour 3, 259–262 (2011). https://doi.org/10.1007/s12686-010-9336-9
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DOI: https://doi.org/10.1007/s12686-010-9336-9