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Anonymous nuclear markers for SouthEast Asian halfbeak fishes (Dermogenys)

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Abstract

We report the development of nine single-copy anonymous nuclear polymorphic loci (SCNPs) developed from the SouthEast Asian freshwater livebearing halfbeak fish Dermogenys pusilla. Ninety-six clones from a genomic library were examined, and 43 potential loci were tested across several species of Dermogenys, and in the related halfbeak Hemirhamphodon pogonognathus. All loci reported here amplified successfully in six putative species of Dermogenys, but not in Hemirhamphodon. An average of 50 SNPs per kilobase were identified (total of 5,850 bp sequenced per individual). These markers should provide a useful resource for species delineation, and other phylogenetic, phylogeographic and population genetic studies of Dermogenys halfbeaks.

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Acknowledgments

We thank Bryan Carstens and Bryce Noonan for helpful advice and protocols. This research was funded by a European Community Marie Curie Incoming International Fellowship (FP6 MIIF-CT-2006-39798) to MdB.

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Authors and Affiliations

  1. School of Biological Sciences, Bangor University, Deiniol Road, Bangor, LL57 2UW, UK

    Mark de Bruyn, Wendy Grail, Axel Barlow & Gary R. Carvalho

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  1. Mark de Bruyn
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  2. Wendy Grail
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  3. Axel Barlow
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  4. Gary R. Carvalho
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Correspondence to Mark de Bruyn.

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de Bruyn, M., Grail, W., Barlow, A. et al. Anonymous nuclear markers for SouthEast Asian halfbeak fishes (Dermogenys). Conservation Genet Resour 2 (Suppl 1), 325–327 (2010). https://doi.org/10.1007/s12686-010-9228-z

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  • DOI: https://doi.org/10.1007/s12686-010-9228-z

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