Abstract
We describe primers and PCR conditions to amplify seven tetranucleotide loci and two dinucleotide loci isolated from the Malagasy poison frog Mantella crocea. In 21 individuals from Ambohitantely Reserve, the number of alleles ranged from 3 to 15, observed heterozygosities from 24 to 95%. No evidence for linkage disequilibrium was found, but four loci deviated significantly from Hardy–Weinberg equilibrium, possibly due to presence of null alleles.
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Acknowledgments
The first part of the microsatellite isolation process was performed while JSH was at the Unit of Evolutionary Genetics at the University of Cologne, and we thank Diethard Tautz for support and Jessika Füssel for her help in the lab. At Cologne, JSH was supported by a postdoctoral fellowship from the Max-Planck Institute for Evolutionary Biology in Plön. AC is grateful to Sabine Hagemann and Heike Pröhl for assistance.
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Hauswaldt, J.S., Crottini, A. & Vences, M. Nine microsatellite loci for the Malagasy Poison Frogs (Mantella). Conservation Genet Resour 1, 269–271 (2009). https://doi.org/10.1007/s12686-009-9066-z
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DOI: https://doi.org/10.1007/s12686-009-9066-z